Summary
A transformation system for Colletotrichum trifolii, a fungal pathogen of alfalfa, has been developed using whole cells as recipients. Hygromycin B and benomyl resistant colonies were isolated following treatment of fungal tissue with lithium acetate and separate plasmids containing the respective genes which confer resistance to these antibiotics. The DNA was stably integrated into the fungal chromosome. This approach to transformation has general utility for phytopathogenic fungi and represents an initial step in the molecular analysis of virulence determinants in this race specific fungus.
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Dickman, M.B. Whole cell transformation of the alfalfa fungal pathogen Colletotrichum trifolii . Curr Genet 14, 241–246 (1988). https://doi.org/10.1007/BF00376744
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DOI: https://doi.org/10.1007/BF00376744