Summary
We have studied the course of the operculum line in the larval hypoderm of several bithorax complex mutants of Drosophila melanogaster. The bifurcation of the line, a characteristic of the first abdominal segment in wild-type (A1), can also appear in the metathoracic (T3) and other abdominal segments (A2, A3) depending on mutations within the bithorax complex. Therefore, we concluded that the course of the operculum line and thus the shape of the operculum is not determined by a suprasegmental gradient of positional information but by the functional state of the genes of the bithorax complex in each metamere. The dorsal and ventral branches of the operculum line react differently, the dorsal branch being more sensitive to the effect of loss of function mutations (bxd, iab-2 k), the ventral branch more affected by gain of function mutations (Hab). In some cases the effects of the mutations on the operculum line differed from those in the adult, suggesting a difference in sensitivity of larval hypodermal cells and histoblast cells to the functional gene products of the bithorax complex.
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References
Akam ME (1983) The location of Ultrabithorax transcripts in Drosophila tissue sections. EMBO J 2:2075–2084
Akam ME, Martinez-Arias A (1985) The distribution of Ultrabithorax transcripts in Drosophila embryos. EMBO J 4:1689–1700
Hayes PH, Sato T, Denell RE (1984) Homoeosis in Drosophila: the ultrabithorax larval syndrome. Proc Natl Acad Sci (USA) 81:545–549
Keilin D (1915) Recherches sur les larves Diptères cyclorraphes. Bull Sci Fr Belg 49:15–198
Kerridge S, Sang JH (1981) Developmental analysis of the homoeotic mutation bithoraxoid of Drosophila melanogaster. J Embryol Exp Morphol 61:69–86
Kuhn DT, Woods DF, Cook JL (1981) Analysis of a new homoeotic mutation (iab-2) within the bithorax complex in Drosophila melanogaster. Mol Gen Genet 181:82–86
Lewis EB (1963) Genes and developmental pathways. Am Zool 3:33–56
Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276:565–570
Lindsley DL, Grell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Institute Washington, Publ. No. 627
Martinez-Arias A, Lawrence PA (1985) Parasegments and compartments in the Drosophila embryo. Nature 313:639–642
McGinnis W, Levine MS, Hafen E, Kuroiwa A, Gehring WJ (1984) A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes. Nature 308:428–433
Minana FJ, Garcia-Bellido A (1982) Preblastoderm mosaics of mutants of the bithorax complex. Wilhelm Roux's Arch 191:331–334
Segal D, Spreij ThE (1984) The dorsal/ventral compartment boundary in Drosophila: coincidence with the prospective operculum seam. Wilhelm Roux's Arch 193:133–138
Struhl G (1984) Splitting the bithorax complex of Drosophila. Nature 308:454–457
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Spreij, T.E., Segal, D. Determination of the shape of the operculum by the bithorax complex in Drosophila melanogaster . Roux's Arch Dev Biol 195, 318–322 (1986). https://doi.org/10.1007/BF00376064
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DOI: https://doi.org/10.1007/BF00376064