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Neurotransmitters inhibit the omega-conotoxin-sensitive component of Ca current in neuroblastoma × glioma hybrid (NG108-15) cells, not the nifedipine-sensitive component

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Abstract

Voltage-dependent calcium currents (I Ca) in NG 108-15 cells consisted of three pharmacologically distinct components: a transient low-voltage-activated (LVA) current, sensitive to Ni2+; a high-voltage-activated (HVA) current sensitive to the dihydropyridine antagonist, nifedipine and a HVA current sensitive to omega-conotoxin GVIA (CgTx). The voltage sensitivities and decay kinetics of the two HVA currents were indistinguishable. The neurotransmitters acetylcholine (ACh) and noradrenaline inhibited I Ca. This inhibition was not occluded by Ni2+ or nifedipine, but was abolished by CgTx. It is therefore concluded that the neurotransmitter-sensitive component of ICa is restricted to that component of HVA current inhibitable by omega-conotoxin.

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Authors and Affiliations

  1. Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, UK

    M. P. Caulfield, J. Robbins & D. A. Brown

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  1. M. P. Caulfield
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  2. J. Robbins
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  3. D. A. Brown
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Caulfield, M.P., Robbins, J. & Brown, D.A. Neurotransmitters inhibit the omega-conotoxin-sensitive component of Ca current in neuroblastoma × glioma hybrid (NG108-15) cells, not the nifedipine-sensitive component. Pflugers Arch. 420, 486–492 (1992). https://doi.org/10.1007/BF00374623

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  • DOI: https://doi.org/10.1007/BF00374623

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