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A novel method for incorporation of ion channels into a planar phospholipid bilayer which allows solution changes on a millisecond timescale

  • Instruments and Techniques
  • Molecular and Cellular Physiology
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Abstract

We have developed a method of rapidly changing the solutions on one side of a planar phospholipid bilayer. Bilayers can be painted on glass pipettes of tip diameter ⩾ 50 μm. By modifying an established method for rapid exchange of solutions bathing excised membrane patches, solution changes can be made at the bilayer within 10 ms. After incorporation of channels into the bilayer, the bilayer is moved into one of two parallel streams of solution flowing from a length of double-barrelled glass theta tubing. Activation of a solenoid system rapidly moves the theta tubing so that the bilayer is in the flow of the adjacent solution. For various reasons, the single-channel gating mechanisms of many channels are studied in planar bilayer systems. The conventional bilayer technique only allows for steady-state single-channel gating to be monitored. This novel method now allows the effects of rapid changes in modulators of channels incorporated into planar phospholipid bilayers to be measured.

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Authors and Affiliations

  1. Department of Cardiac Medicine, The National Heart and Lung Institute, Dovehouse Street, SW3 6LY, London, UK

    Rebecca Sitsapesan, Richard A. P. Montgomery & Alan J. Williams

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  1. Rebecca Sitsapesan
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  2. Richard A. P. Montgomery
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  3. Alan J. Williams
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Sitsapesan, R., Montgomery, R.A.P. & Williams, A.J. A novel method for incorporation of ion channels into a planar phospholipid bilayer which allows solution changes on a millisecond timescale. Pflugers Arch. 430, 584–589 (1995). https://doi.org/10.1007/BF00373896

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  • DOI: https://doi.org/10.1007/BF00373896

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