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Inhibition of lipase-catalyzed hydrolysis of sunflower oil in AOT/isooctane reversed micelles

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Abstract

The hydrolysis of sunflower oil using Candida cylindracea lipase in reversed micelles of AOT/isooctane was investigated. The inhibition caused by substrate and hydrolysis products has been found in the process of reaction. It was revealed that the extent of inhibition caused by oleic acid was higher than that caused by glycerol, and was much more serious in the case of the mixture of hydrolysis products. Moreover, with the initial addition of glycerol into the reaction mixture, the stability of lipase could be increased during the hydrolysis of sunflower oil in reversed micelles.

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References

  1. Han, D.; Rhee, J. S.: Batchwise hydrolysis of olive by lipase in AOT-isooctane reversed micelles. Biotechnol. Lett. 7 (1985) 651–656

    Article  CAS  Google Scholar 

  2. Kim, T.; Chung, K.: Some characteristics of palm kernel olein hydrolysis by Rhizopus arrhizus lipase in reversed micelle of AOT in isooctane, and additive effects. Enzyme Microb. Technol. 11 (1989) 528–532

    Article  CAS  Google Scholar 

  3. Prazeres, D. M. F.; Garcia, F. A. P.; Cabral, J. M. S.: An ultrafiltration membrane bioreactor for the lipolysis of olive oil in reversed micellar media. Biotech. Bioeng. 41 (1993) 761–770

    Article  CAS  Google Scholar 

  4. Prazeres, D. M. F.; Lemos, F.; Garcia, F. A. P.; Cabral, J. M. S.: Modeling lipolysis in a reversed micellar system: Part I-Conventional batch reactor. 1993. Biotech. Bioeng. 42 (1193) 759–764

    Article  Google Scholar 

  5. Tsai, S. W.; Chiang, C. L.: Kinetics, mechanism, and time course analysis of lipase-catalyzed hydrolysis of high concentration olive oil in AOT-isooctane reversed micelles. Biotech. Bioeng. 38 (1991) 206–209

    Article  CAS  Google Scholar 

  6. Han, D.; Rhee, J. S.; Lee, S. B.: Lipase reaction in AOT-isooctane reversed micelles: Effect of water on equilibria. Biotech. Bioeng. 30 (1987) 381–388

    Article  CAS  Google Scholar 

  7. Prazeres, D. M. F.; Lemos, F.; Garcia, F. A. P.; Cabral, J. M. S.: Modeling lipolysis in a reversed micellar system: Part II-Membrane reactor. Biotech. Bioeng. 42 (1993) 765–771

    Article  CAS  Google Scholar 

  8. Lowery, R. R.; Tinsely, I. J.: A simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J. Am. Oil Chem. Soc. 53 (1976) 470–473

    Google Scholar 

  9. Malcata, F. X.; Reyes, H. R.; Garcia, H. S.; Hill Jr., C. G.; Amundson, C. H.: Kinetics and mechanisms of reaction catalyzed by immobilized lipases. Enzyme Microb. Technol. 14 (1992) 426–446

    Article  CAS  Google Scholar 

  10. Derksen, J. T. P.; Cuperus, F. P.: Lipase-catalyzed hydrolysis of crambe oil in AOT-isooctane reversed micelles. Biotechnol. Lett. 14 (1992) 937–940

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Chemical Metallurgy, Chinese Academy of Sciences, P.O. Box 353, 100080, Beijing, China

    Q -L. Chang & J -Y. Chen

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  1. Q -L. Chang
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  2. J -Y. Chen
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We thank the National Natural Science Foundation of China for the financial support of this work. We also thank Prof. Xu, Jia-li for his contributions to this work.

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Chang, Q.L., Chen, J.Y. Inhibition of lipase-catalyzed hydrolysis of sunflower oil in AOT/isooctane reversed micelles. Bioprocess Eng. 13, 157–159 (1995). https://doi.org/10.1007/BF00369699

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  • DOI: https://doi.org/10.1007/BF00369699

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