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Enzymatic reaction of ethanol and oleic acid by lipase and lignin peroxidase in rhamnolipid (RL) reversed micelles

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Abstract

An environment friendly bio-surfactant of rhamnolipid (RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase (lip) was evaluated. The optimum conditions of enzymatic reaction catalyzed by lipase (lip) were water to amphiphile molar ratio of 30 (20), RL of 60 (60) critical micelle concentration (CMC), pH of 7.0 (3.0) and temperature of 40 (30) °C, respectively. The change of enzyme conformation indicates that, for catalytic of lipase, water content is the most important factor of the enzymatic reaction of oleic acid, and pH for lip. With individual optimum conditions, the enzymatic efficiency of oleic acid catalyzed by lipase is higher than that by lip. In the presence of ethanol, the enzymatic reaction of oleic acid catalyzed by lipase suits Ping-Pong Bi-Bi mechanism. As an alternative to chemical reversed micelles, the RL reversed micelles are promising methods to enzymatic reaction of oleic acid.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China

    Shan Bao  (包珊), Xing-zhong Yuan  (袁兴中), Xin Peng  (彭馨), Guang-ming Zeng  (曾光明), Hai-peng Wu  (武海鹏), Hou Wang  (王侯), Huan Liu  (刘欢), Yue-Jie Ma  (马玉洁), Kai-long Cui  (崔凯龙) & Xiu-lian Wu  (吴秀莲)

  2. Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education (Hunan University), Changsha, 410082, China

    Shan Bao  (包珊), Xing-zhong Yuan  (袁兴中), Xin Peng  (彭馨), Guang-ming Zeng  (曾光明), Hai-peng Wu  (武海鹏), Hou Wang  (王侯), Huan Liu  (刘欢), Yue-Jie Ma  (马玉洁), Kai-long Cui  (崔凯龙) & Xiu-lian Wu  (吴秀莲)

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  1. Shan Bao  (包珊)
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  2. Xing-zhong Yuan  (袁兴中)
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  3. Xin Peng  (彭馨)
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  4. Guang-ming Zeng  (曾光明)
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  5. Hai-peng Wu  (武海鹏)
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  6. Hou Wang  (王侯)
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  7. Huan Liu  (刘欢)
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  8. Yue-Jie Ma  (马玉洁)
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  9. Kai-long Cui  (崔凯龙)
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  10. Xiu-lian Wu  (吴秀莲)
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Corresponding author

Correspondence to Xing-zhong Yuan  (袁兴中).

Additional information

Foundation item: Projects(50978087, 51009063, 50978088) supported by the National Natural Science Foundation of China

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Bao, S., Yuan, Xz., Peng, X. et al. Enzymatic reaction of ethanol and oleic acid by lipase and lignin peroxidase in rhamnolipid (RL) reversed micelles. J. Cent. South Univ. 22, 2936–2944 (2015). https://doi.org/10.1007/s11771-015-2829-7

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  • DOI: https://doi.org/10.1007/s11771-015-2829-7

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