Abstract
The authors have developed a continuous recycle reactor which efficiently performs emulsion type enzymatic reactions. The reactor column is filled with immobilised lipase and the reactions are effected by pumping the pre-prepared oil-water emulsion through the bottom of the reactor. A part of the product was recycled back and this type of recycling greatly improves the productivity of fatty acid compared to continuous once-through reactor without recycling. The recycle reactor could be continuously run for 35 days without decrease in conversions. The performance of the reactor was interpreted by a model and the theoretical conversion was compared with the experimental data.
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Abbreviations
- F AO μmol/min:
-
feed rate
- K M g/l:
-
Michaelis constant
- R :
-
recycle ratio
- r 5 μmol/(ml · min):
-
reaction rate
- S 0 g/l:
-
initial substrate concentration
- V max μmol/(ml · min):
-
maximum reaction velocity
- V R l:
-
void volume of the reactor
- x s :
-
fractional conversion
- Standard deviation:
-
\(\left( {{{\left[ {\mathop \Sigma \limits_1^n \left\{ {\left( {C_{exp} - C_{model} } \right)/C_{exp} } \right\}^2 } \right]} \mathord{\left/ {\vphantom {{\left[ {\mathop \Sigma \limits_1^n \left\{ {\left( {C_{exp} - C_{model} } \right)/C_{exp} } \right\}^2 } \right]} {(n - 1)^{0.5} }}} \right. \kern-\nulldelimiterspace} {(n - 1)^{0.5} }}} \right)\)
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Padmini, P., Rakshit, S.K., Krishnaiah, K. et al. Studies on a continuous recycle reactor for a lipase-catalysed processing of ricebran oil. Bioprocess Engineering 11, 39–42 (1994). https://doi.org/10.1007/BF00369613
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DOI: https://doi.org/10.1007/BF00369613