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Effect of mode of operation of bioreactors on the biosynthesis of penicillin amidase in Escherichia coli

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Abstract

Different operational mode of bioreactors influence the biosynthesis of the enzyme and related products as well as the growth of industrial microorganisms. This communication deals with the effect of mode of operation of various bioreactors with different geometric configurations, viz., batch (includes commercially available batch stirred tank, and custom-designed cylindrical and tapered reactors), batch-fed, continuous flow stirred tank reactors on the biosynthesis of penicillin amidase in Escherichia coli. Experimental findings show that the biosynthesis of penicillin amidase in E. coli show a little variation among batch reactor modes and significant variation on the continuous mode of operation. Further analysis show that the different reactor modes also influence periplasmic localization of the enzyme in the cell.

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References

  1. Shewale, J.G.;Deshpande, B.S.;Sudhakaran, V.K.;Ambedkar, S.S.: Penicillin acylases: Applications and Potentials. Process Biochem. 25 (1990) 97–103

    Google Scholar 

  2. Illanes, A.;Acevedo, F.;Gentina, J.C.;Reyes, I.;Torres, R.;Cartagena, O.;Ruiz, A.;Vasquez, M.: Production of penicillin acylase from Bacillus megaterium in complex and defined media. Process Biochem. 29 (1994) 263–270

    Google Scholar 

  3. Cooney, C.L.: Growth of microorganisms. In: Biotechnology Vol. I, p. 75 (Rehm, H.-J., Reed, G., eds.). Verlag Chemie, Weinheim, 1981

    Google Scholar 

  4. Babu, P.S.R.;Panda, T.: The role of phenylacetic acid in biosynthesis of penicillin amidase in E. coli. Bioproc. Eng. 6 (1991) 71–74

    Google Scholar 

  5. Babu, P.S.R.;Panda, T.: Studies on improved techniques for immobilizing and stabilizing penicillin amidase associated with E. coli cells. Enzyme Microb. Technol. 13 (1991) 676–682

    Google Scholar 

  6. Mallette, M.F.: Evaluation of growth by physical and chemical means. In: Methods in Microbiology. Vol. I, pp. 521–526 (Norris, J.S., Ribbons, D.W., eds.) Academic Press, London, 1969

    Google Scholar 

  7. Babu, P.S.R.: Studies on biosynthesis of penicillin amidase in Escherichia coli, stabilization and immobilization of the enzyme associated with whole cells. Ph.D. Thesis, Indian Institute of Technology, pp. 331–333, 1991

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Authors and Affiliations

  1. Biotechnology Research Centre, Department of Chemical Engineering, Indian Institute of Technology, 600 036, Madras, India

    K. Jagannadha Rao & T. Panda

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  1. K. Jagannadha Rao
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  2. T. Panda
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Rao, K.J., Panda, T. Effect of mode of operation of bioreactors on the biosynthesis of penicillin amidase in Escherichia coli. Bioprocess Engineering 14, 317–321 (1996). https://doi.org/10.1007/BF00369476

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  • DOI: https://doi.org/10.1007/BF00369476

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