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Comparison of different bioreactor performances

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Abstract

Bioreactors are compared based on oxygen transfer rate and efficiency, mixing performance, cell mass productivity as well as with respect to enzyme and metabolite productivity.

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Abbreviations

AC:

acetate concentration

AL:

airlift tower loop reactor

CFU:

colony-forming units

CP:

coalescence-promoting medium

CS:

coalescence-suppressing medium

D D :

impeller clearance

D M :

molecular diffusivity

D S :

diameter of the column

DT:

flat-bladed disc turbine

D v :

vessel diameter

E. act:

enzyme activity

EDR:

energy dissipation rate

EcoRI:

restriction endonuclease

EcoR4:

protection plasmid

E O 2 :

efficiency of oxygen transfer rate

E X :

efficiency of cell mass production with respect to the specific power input

g :

acceleration of gravity

H :

height of column

H v :

vessel height

HV:

highly viscous medium

IPTG:

isopropyl thiogalactoside (inducer of Lacpromoter)

k :

fluid consistency factor

k L :

mass transfer coefficient

k La:

volumetric mass transfer coefficient

m :

exponent

N :

impeller speed

n :

exponent

n :

flow behaviour index

P :

power input

P/VL :

specific power input

PR:

marine propeller

P LacUV5 :

Lac-promoter-induced by IPTG

P R :

promoter-induced with temperature shift

ϱ O 2 :

oxygen transfer rate

q g,q O 2 :

aeration rate, specific aeration rate with respect to liquid volume

R :

density of cultivation medium

R p :

product formation rate

R X :

growth rate

SpA:

protein A

ST:

stirred tank reactor

TCC:

total cell count

t Lc :

liquid circulation time

U :

enzyme activity unit

u B :

bubble rise velocity

u G :

superficial gas velocity

V L :

volume of the liquid phase

v :

kinematic viscosity of the cultivation medium

W SG :

superficial gas velocity

X :

cell mass concentration

Y E/S :

yield coefficient of ethanol formation with respect to substrate consumption

Y P/X :

specific product formation with respect to cell concentration

Y X/E :

yield coefficient of cell growth with respect to ethanol consumption

Y X/O 2 :

yield coefficient of cell growth with respect to oxygen consumption rate

Y X/S :

yield coefficient of growth with respect to substrate consumption

θ L :

liquid mixing time

μ eff :

effective dynamic viscosity of the cultivation medium

μ W :

dynamic viscosity of water

μ max :

maximum specific growth rate

σ :

surface tension of the cultivation medium

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  1. Institut für Technische Chemie, Universität Hannover, Callinstr. 3, D-30167, Hannover, FRG

    K. Schügerl

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Schügerl, K. Comparison of different bioreactor performances. Bioprocess Engineering 9, 215–223 (1993). https://doi.org/10.1007/BF00369405

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