Abstract
Kinetics of enzymatic hydrolysis of starch to high maltose syrup (by simultaneous use of β-amylase and isoamylase) has been studied here. Main product of dual-enzyme system, maltose, showed a competitive inhibition effect on apparent overall activity of enzymes. Thermal inactivation behavior could be expressed by an empirical exponential function. A mathematical model developed here has described performance of an ultrafiltration reactor (UFR) system by considering effects of product inhibition, enzyme deactivation, and formation of side-product. Effects of concentrations in substrate and enzymes, with residence time of substrate on the performance of UFR has been investigated. Proposed model has been successfully verified in simulating experimental data under various conditions. Operation stability of UFR has also been studied.
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Houng, J.Y., Chiou, J.Y. & Chen, K.C. Production of high maltose syrup using an ultrafiltration reactor. Bioprocess Eng. 8, 85–90 (1992). https://doi.org/10.1007/BF00369269
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DOI: https://doi.org/10.1007/BF00369269