Skip to main content
SpringerLink
Log in

Production of high maltose syrup using an ultrafiltration reactor

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Kinetics of enzymatic hydrolysis of starch to high maltose syrup (by simultaneous use of β-amylase and isoamylase) has been studied here. Main product of dual-enzyme system, maltose, showed a competitive inhibition effect on apparent overall activity of enzymes. Thermal inactivation behavior could be expressed by an empirical exponential function. A mathematical model developed here has described performance of an ultrafiltration reactor (UFR) system by considering effects of product inhibition, enzyme deactivation, and formation of side-product. Effects of concentrations in substrate and enzymes, with residence time of substrate on the performance of UFR has been investigated. Proposed model has been successfully verified in simulating experimental data under various conditions. Operation stability of UFR has also been studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Martensson, K.: Preparation of an immobilized two-enzyme, β-amylase-pullulanase, to an acrylic copolymer for the conversion of starch to maltose. II. Cocoupling of the enzymes and use in a packed bed column. Biotechnol. Bioeng. 17 (1974) 579–591

    Google Scholar 

  2. Reilly, P. J.: Potential and use of immobilized carbohydrases. In: Pitcher, H. W. (Ed.): Immobilized enzymes for food processing, pp. 113–151. Florida: CRC Press, Inc. 1980

    Google Scholar 

  3. Hartmeier, W.: Immobilized biocatalysts-from simple to complex systems. Trends Biotechnol. 3 (1985) 149–153

    Google Scholar 

  4. Ohlson, I.; Tragardh, G.; Hahn-Hagerdal, B.: Enzymatic hydrolysis of sodium hydroxide-pretreated sallow in an ultrafiltration membrane reactor. Biotechnol. Bioeng. 26 (1984) 647–653

    Google Scholar 

  5. Ohshima, T.; Wandrey, C.; Kula, M. R.; Soda, K.: Improvement for L-leucine production in a continuously operated enzyme membrane reactor. Biotechnol. Bioeng. 27 (1985) 1616–1618

    Google Scholar 

  6. Darnoko, D.; Cheryan, M.; Artz, W. E.: Saccharification of cassava starch in an ultrafiltration reactor. Enzyme Microb. Technol. 11 (1989) 154–159

    Google Scholar 

  7. Martensson, K.: Preparation of an immobilized two-enzyme system, β-amylase-pullulanase, to an acrylic copolymer for the conversion of starch to maltose. III. Process kinetic studies on continuous reactors. Biotechnol. Bioeng. 16 (1974) 1567–1587

    Google Scholar 

  8. Shiraishi, F.; Kawakami, K.; Yuasa, A.; Kojima, T.; Kusunoki, K.: Kinetic expression for maltose production from soluble starch by simultaneous use of β-amylase and debranching enzymes. Biotechnol. Bioeng. 30 (1987) 374–380

    Google Scholar 

  9. Houng, J. Y.; Chen, K. C.; Hsu, W. H.: Optimization of cultivation medium composition for isoamylase production. Appl. Microbiol. Biotechnol. 31 (1989) 61–64

    Google Scholar 

  10. Yokobayashi, K.; Misaki, A.; Harada, T.: Purification and properties of Pseudomonas isoamylase. Biochim. Biophys. Acta 212 (1970) 458–469

    Google Scholar 

  11. Houng, J. Y.; Chiou, J. Y.; Chen, K. C.; Hsu, W. H.: Characteristics of starch hydrolysis using β-amylase and isoamylase. J. Chinese Agric. Chem. Soc. 29 (1991) 54–60

    Google Scholar 

  12. Hollo, J. E.; Laszlo, E.; Hoschke, A.: Mechanism of amylolytic starch degradation. Die Stärke 25 (1973) 1–36

    Google Scholar 

  13. Kitagawa, H.; Amemura, A.; Harada, T.: Studies on the inhibition and molecular properties of crystalline Pseudomonas isoamylase. Agric. Biol. Chem. 39 (1975) 989–994

    Google Scholar 

  14. Davis, B. J.: Disc electrophoresis — II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121 (1964) 404

    Google Scholar 

  15. Closset, G. P.; Cobb, J. T.; Shah, Y. T.: Study of performance of a tubular membrane reactor for an enzyme catalyzed reaction. Biotechnol. Bioeng. 16 (1974) 345–360

    Google Scholar 

  16. Madgavkar, A. M.; Shah, Y. T.; Cobb, J. T.: Hydrolysis of starch in a membrane reactor. Biotechnol. Bioeng. 19 (1977) 1719–1726

    Google Scholar 

  17. Hong, G. T.; Tsao, T.; Wankat, P. C.: Membrane reactor for enzymatic hydrolysis of cellobiose. Biotechnol. Bioeng. 23 (1981) 1501–1516

    Google Scholar 

  18. Gianfreda, L.; Livosi, A. M.; Scarfi, M. R.; Greco Jr., G.: β-D-Glucosidase stabilization in a polarized ultrafiltration membrane reactor. Enzyme Microb. Technol. 4 (1982) 322–326

    Google Scholar 

  19. Larsson, M.; Mattiasson, B.: Continuous conversion of starch to ethanol using a combination of an aqueous two-phase system and an ultrafiltration unit. In: Laskin, A. I.; Tsao, G. T.; Wingard Jr., L. B. (Eds.): Enzyme engineering, vol. 7, pp. 144–147. New York: New York Academy of Sciences 1984

    Google Scholar 

  20. Gianfreda, L.; Pirozzi, D.; Greco Jr., G.: Microenvironmental effect of stabilizing polyelectrolytes in ultrafiltration membrane enzymatic reactors. Biotechnol. Bioeng. 33 (1989) 1067–1071

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 30043, Taiwan, Republic of China

    J. -Y. Houng, J. -Y. Chiou &  K. -C. Chen

Authors
  1. J. -Y. Houng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. -Y. Chiou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. -C. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Houng, J.Y., Chiou, J.Y. & Chen, K.C. Production of high maltose syrup using an ultrafiltration reactor. Bioprocess Eng. 8, 85–90 (1992). https://doi.org/10.1007/BF00369269

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00369269

Keywords

Search

Navigation