Abstract
A simple high density cell culture system is described which demonstrates many of the features of commercially available hollow-fibre systems, but without the need to invest in a dedicated system. The system has been shown to achieve product concentrations of up to 40-fold greater than that obtained in batch culture making gram production of MAbs possible with considerable saving on serum costs.
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Abbreviations
- ELISA :
-
enzyme-linked immunosorbent assay
- FBS :
-
foetal bovine serum
- IgG :
-
immunoglobulin G
- IgM :
-
immunoglobulin M
- MAb :
-
monoclonal antibody
- pH:
-
negative common logarithm of the hydrogen ion concentration
- pO2 :
-
dissolved oxygen concentration
- RPMI 1640 :
-
Roswell Park Memorial Institute medium 1640
References
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Knazek, R. A.; Gullino, P. M.; Kohler, P. O.; Dedrick, R. L.: Cell culture on artificial capillaries. Science 178 (1972) 65–66
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Munster, M.J., Kearns, M.J., Steegmans, U. et al. A high density culture system for the in-vitro production of human and mouse monoclonal antibodies. Bioprocess Engineering 6, 123–125 (1991). https://doi.org/10.1007/BF00369065
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DOI: https://doi.org/10.1007/BF00369065