Abstract
The half-life of the indian ink elimination (carbon clearance method) was used as a measure of the RES activity. A significant increase of the RES activity in mice was found already after a single intraperitoneal (i.p.) administration of 20, 50, or 100 μg PbAc/kg body weight (b.w.). A significant RES stimulation could also be demonstrated after a single or 10-day oral administration of lead at doses of 10–1,000 μg PbAc/kg b.w. No RES stimulation occurred, however, after 30-day oral administration of lead. On determining the reactivity of the RES to 1 μg endotoxin/kg b.w. it was found that it was already limited after a single and 10-day oral lead administration. After 30-day oral administration of lead (doses 10–1,000 μg PbAc/kg b.w.) the reactivity of the RES was completely suppressed.
After a single and 10-day oral administration of lead (doses 10–1,000 μg PbAc/kg b.w.) we observed a marked leucocytosis. This effect was most clearly seen after a dose of 100 μg PbAc/kg b.w.; the leucocyte counts were increased by up to 50% in comparison with controls. After 30-day oral administration of lead, such a leucocytosis was no longer detectable. In accordance with the findings on the RES, the leucocytosis that is normally induced in the animals by 1 μg endotoxin/kg b.w. was significantly reduced or completely suppressed both after single and also after 10 and 30 days' oral administration of lead at doses of 100 and 1,000 μg PbAc/kg b.w. These results show that the resistance state of the mouse is impaired already by low doses of lead.
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References
Biozzi G, Benacerraf B, Halpern BN (1953) Quantitative study of the granulopectic activity of the reticulo-endothelial system II. Br J Exp Pathol 34: 441–457
Bridges JB, McClure J (1972) Experimental calcification in a number of species. Calc Tissue Res 10: 136–141
Cook JA, Di Luzio NR (1973) Protective effect of cysteine and methylprednisolone in lead acetate-endotoxin induced shock. Exp Mol Pathol 19: 127–138
Dunnett CW (1955) A multiple comparison procedure for comparing several treatments with a control. JASA 50: 1096–1121
Faith RE, Luster MI, Kimmel CA (1979) Effect of chronic developmental lead exposure on cellmediated immune functions. Clin Exp Immunol 35: 413–420
Gaworski CL, Sharma RP (1978) The effects of heavy metals on [3H]-thymidine uptake in lymphocytes. Toxicol Appl Pharmacol 46: 305–313
Goyer RA, Moore JF (1972) Cellular effects of lead. Adv Exp Med 48: 447–462
Hogan GR (1977) Single lead acetate insult, testosterone therapy, and erythropoiesis in mice. J Toxicol Environ Health 3: 577–588
Hogan GR, Adams DP (1979) Lead induced leucocytosis in female mice. Arch Toxicol 41: 295–300
Jones RB, Wiese JL, Kiesow LA (1973) Failure of methylprednisolone to protect lead sensitized rats against endotoxin. Infect Immun 8: 683–684
Koller LD (1973) Immunosuppression produced by lead, cadmium, and mercury. Am J Vet Res 34: 1457–1458
Koller LD, Kovacic S (1974) Decreased antibody formation in mice exposed to lead. Nature 250: 148–150
Koller LD, Brauner JA (1977) Decreased b-lymphocyte response after exposure to lead and cadmium. Toxicol Appl Pharmacol 42: 621–624
Krüger W (1973) Die Stimulierung des RES der Maus durch ein-, zweiund fünffache Applikation von Endotoxin. Inaugural-Dissertation, Medizin. Fakultät Göttingen, FRG
Metzger GV, Casarrett LJ (1969) Influence of particle and cell concentration on phagocytosis of Cr2O3 by peritoneal macrophages. J Reticuloendothel Soc 6: 435–447
Mitema ES, Oehme FW, Penumarthy L, Moore WE (1980) Effect of chronic lead exposure on the canine bone marrow. Am J Vet Res 41: 682–685
Reigart JR, Graber CD (1976) Evaluation of the humoral immune response of children with low level lead exposure. Bull Environ Contam Toxicol 16: 112–117
Schlick E, Kamran MA, Friedberg KD (1979) The influence of minimal doses of lead on d-ala-d, res and leucocytes of mice. Arch Pharmacol 308: R48
Schlipköter HW, Ghelerter L, Sonderfeld M (1976) Der Einfluß von Blei auf die Antikörperbildung. Zentralbl Bakteriol [Orig B] 163: 417–424
Selye H, Tuchweber B, Bertok L (1966) Effect of lead acetate on the susceptibility of rats to bacterial endotoxins. J Bacteriol 91: 884–890
Sroczynski J, Jonderko G, Watras J (1965) Untersuchungen über die Lebensdauer der roten Blutkörperchen mittels radioaktivem Chrom (Cr51) bei experimenteller Bleivergiftung und bei prophylaktischer Anwendung von CaNa2 EDTA. Int Arch Gewerbepathol Gewerbehyg 21: 223–230
Stole V (1975) Effect of calcium and lanthanum on iodine accumulation and release from human polymorphonuclear leucocytes. J Reticuloendothel Soc 18: 213–220
Trejo RA, Di Luzio NR, Loose LD, Hoffman E (1972) Reticuloendothelial and hepatic functional alterations following lead acetate administration. Exp Mol Pathol 17: 145–158
Wilkins DJ, Bangham AD (1964) The effect of some metal ions on in vitro phagocytosis. J Reticuloendothel Soc 1: 233–242
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Schlick, E., Friedberg, K.D. The influence of low lead doses on the reticulo-endothelial system and leucocytes of mice. Arch Toxicol 47, 197–207 (1981). https://doi.org/10.1007/BF00368680
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DOI: https://doi.org/10.1007/BF00368680