Abstract
GpppG was modified to m7GpppGm by a cytoplasmic extract, prepared from embryonic lens cells, in a reaction mixture which contained S-adenosyl-methionine as methyl group donor. The appearance of m7GpppGm was a function of time and lens extract concentration. S-adenosyl-homocysteine inhibited both the m7G and Gm modification reactions. Analogues of GpppG, pG, ppG and pppG were relatively ineffective as substrates.
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Lavers, G.C. Detection of methyltransferase activities which modify Gppp G TO m7GpppGm in embryonic chick lens. Molecular Biology Reports 3, 275–281 (1977). https://doi.org/10.1007/BF00368297
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DOI: https://doi.org/10.1007/BF00368297