Skip to main content
SpringerLink
Log in

Template active chromatin structures: Degradation by deoxyribonuclease I

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Chicken embryos were pulse-labelled in vivo with [3H]uridine (10 min), the chromatin isolated and treated with DNAse I. The residual chromatin was separated from the degradation products by centrifugation. The nascent pulse-labelled RNA is completely recovered in the residual chromatin even after prolonged incubation with DNAase I, whereas the DNA is completely degraded to 80 base polynucleotide fragments and smaller fragments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

cDNA:

DNA complementary to mRNA

DOC:

deoxycholate

EDTA:

ethylendiamintetraacetic acid

SDS:

sodium-dodecylsulfate

References

  1. Mirsky, A. E., Silverman, B., and Panda, N. C., Proc. Nat. Acad. Sci. U.S.A. 69, 3243 (1972).

    Google Scholar 

  2. Clark, R. J., and Felsenfeld, G., Biochemistry 13, 3622 (1974).

    Google Scholar 

  3. Noll, M., Nature 251, 249 (1974).

    Google Scholar 

  4. Hewish, D. R., and Burgoyne, A. L., Biochem. Biophys. Res. Comm. 52, 504 (1973).

    Google Scholar 

  5. Olins, A. L., Carlson, R. D., and Olins, D. E., J. Cell. Biol. 64, 528 (1975).

    Google Scholar 

  6. Felsenfeld, G., Nature 257, 177 (1975).

    Google Scholar 

  7. Noll, M., Nucleic Acid Research 1, 1573 (1974).

    Google Scholar 

  8. Lacy, E., and Axel, R., Proc. Nat. Acad. Sci. U.S.A. 72, 3978 (1975).

    Google Scholar 

  9. Tien Kuo, M., Sahasrabuddhe, C. G., and Saunders, G. F., Proc. Nat. Acad. Sci. U.S.A. 73, 1572 (1976).

    Google Scholar 

  10. Cheveau, J., Moule, Y., and Rouiller, Ch., Exptl. Cell Res. 11, 317 (1956).

    Google Scholar 

  11. Knöchel, W., and Tiedemann, H., Biochim. Biophys. Acta 269, 104 (1972).

    Google Scholar 

  12. Berns, K. I., and Thomas, C. A. Jr., J. Mol. Biol. 11, 476 (1965).

    Google Scholar 

  13. Tiedemann, H., Born, J., and Tiedemann, H., Wilhelm Roux' Archiv 171, 169 (1972).

    Google Scholar 

  14. Scherrer, K., and Darnell, J. E., Biochem. Biophys. Res. Comm. 7, 486 (1962).

    Google Scholar 

  15. Studier, W. F., J. Mol. Biol. 11, 373 (1965).

    Google Scholar 

  16. Mauritzen, Ch. M., Starbuck, W. C., Saroja, I. S., Taylor, Ch. W., and Busch, H., J. Biol. Chem. 242, 2240 (1967).

    Google Scholar 

  17. Penman, Sh., Vesco, C., and Penman, M., J. Mol. Biol. 34, 49 (1968).

    Google Scholar 

  18. Ilyin, Y. V., Varshavsky, A. Ya., Miehelsaar, U. V., and Georgiev, G. P., Europ. J. Biochem. 22, 235 (1971).

    Google Scholar 

  19. Noll, M., Nucleic Acid Research 1, 1573 (1974).

    Google Scholar 

  20. Sentenac, A., Ruet, A., and Fromageot, P., FEBS Letters 2, 53 (1968).

    Google Scholar 

  21. Heyden, B., Nüsslein, Ch., and Schaller, H., Nat. New Biol. 240, 9 (1972).

    Google Scholar 

  22. Weintraub, H., and Groudine, M., Science 193, 848 (1976).

    Google Scholar 

  23. Garel, A., and Axel, R., Proc. Nat. Acad. Sci. U.S.A. 73, 3966 (1976).

    Google Scholar 

  24. Matsuda, G., Maita, T., Mizuno, K., and Ota, H., Nat. New Biol. 244, 244 (1973).

    Google Scholar 

  25. Ross, J., J. Mol. Biol. 106, 403 (1976).

    Google Scholar 

  26. Curtis, P. J., and Weissmann, C., J. Mol. Biol. 106, 1061 (1976).

    Google Scholar 

  27. Knöchel, W., and Grundmann, U., Biochim. Biophys. Acta (1977) in press.

  28. Coutelle, Ch., Ryshov, A. P., and Georgiev, G. P., FEBS Letters 12, 21 (1970).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany

    W. Knöchel, K. Asahi & H. Tiedemann

Authors
  1. W. Knöchel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Asahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Tiedemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Knöchel, W., Asahi, K. & Tiedemann, H. Template active chromatin structures: Degradation by deoxyribonuclease I. Molecular Biology Reports 3, 269–274 (1977). https://doi.org/10.1007/BF00368296

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00368296

Keywords

Search

Navigation