Abstract
Routine determination of IgG and IgM levels is likely to be included in future international immunotoxicity assessment guidelines with the rat as the required animal species. Only a few historical data are available on these two parameters in the rat. We describe a quantitative ELISA sandwich method using commercially available purified rat immunoglobulin as standards and provide the normal range for IgG and IgM values in healthy untreated 10 ± 2 to 30 ± 3 weekold Sprague-Dawley rats. Our data indicate that both IgM and IgG levels increase gradually with age. In addition, we determined IgG values in rats hyperimmunised with protamine, lysozyme and casein.
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Salauze, D., Serre, V. & Perrin, C. Quantification of total IgM and IgG levels in rat sera by a sandwich ELISA technique. Comp Haematol Int 4, 30–33 (1994). https://doi.org/10.1007/BF00368263
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DOI: https://doi.org/10.1007/BF00368263