Abstract
Viability of spores of Bacillus cereus was totally inhibited at 85°C over 30 min by adding 0.4% (w/v) potassium sorbate with 6% (w/v) NaCl at pH 4.5. Viability of B. stearothermophilus spores was totally inhibited at 95°C for 45 min in a buffer at pH 4.2 containing 0.8% (w/v) potassium sorbate and 8% (w/v) NaCl. A synergistic inhibitory effect was demonstrated in some of the combinations. The inhibition may be due to interference with the heat-resistance apparatus of the spores.
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References
BloucherJ.C., BustaF.F. & SofosJ.N. 1982 Influence of potassium sorbate and pH on ten strains of Type A & B Clostridium botulinum. Journal of Food Science 47, 2028–2032.
CameronM.S., LeonardS.J. & BarrettE.L. 1980 Effect of moderately acidic pH on heat resistance of Clostridium sporogenes in phosphate buffer and in buffered pea puree. Applied and Environmental Microbiology 39, 943–949.
FarmiloeF.J., CornfordS.J., CoppockJ.B.M. & IngramM. 1954 The survival of Bacillus subtilis spores in the baking of bread. Journal of the Science of Food and Agriculture 5, 292–304.
GombasD.E. 1983 Bacterial spore resistance to heat. Food Technology 37, 105–110.
GouldG.W., BrownM.H. & FletcherB.C. 1983 Mechanisms of food preservation procedures. In Food Microbiology: Advances and Prospects, eds RobertsT.A. & SkinnerF.A. pp. 67–84. London: Academic Press.
LeistnerL. & RodelW. 1978 Microbiology of intermediate moisture foods. In Proceedings of the International Meeting on Food Microbiology and Technology, eds JarvisB. ChristianJ.H.B. & MicherH.D. pp. 35–56. Parma: Medicina Viva Servizo Congress.
Mallidis, C. 1981 Relationship between heat resistance and chemical composition of bacterial spores. PhD Thesis. University of Strathclyde, Glasgow, UK.
Oloyede, O.B. 1985 The effects of combined preservative systems on bacterial spores and non-sporeformers. PhD Thesis. University of Strathclyde, Glasgow, UK.
OlsonJ.C.Jr & NottinghamP.M. 1980 Factors affecting life and death of microorganisms. In Microbial Ecology of Foods, Vol. 1, ed SillikerJ.H. & ElliotR.P. p. 1. London: ICSMF Academic Press.
SinskeyA.J. 1980 Mode of action and effective application. In Developments in Food Preservatives, Vol. 1, ed TilburyR.H. pp. 111–136. London: Applied Science.
SmootL.A. & PiersonM.D. 1982 Inhibition and control of bacterial spore gemination. Journal of Food Protection 45, 84–92.
SofosJ.N. & BustaF.F. 1983 Sorbates. In Antimicrobials in Foods, eds BranenA.L. & DavidsonP.M. pp. 141–175. New York: Marcel Dekker.
TilburyR.H. 1980 Introduction. In Developments in Food Preservatives, Vol. 1, ed TilburyR.H. pp. 1–24. London: Applied Science.
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O.B. Oloyede was and J. Scholefield is with the Department of Bioscience & Biotechnology, Food Science Division, University of Strathclyde, Glasgow G1 1SD, UK. O.B. Oloyede is now with the Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
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Oloyede, O.B., Scholefield, J. Inhibition of Bacillus spores by combinations of heat, potassium sorbate, NaCl and pH. World J Microbiol Biotechnol 10, 579–582 (1994). https://doi.org/10.1007/BF00367672
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DOI: https://doi.org/10.1007/BF00367672