Skip to main content
SpringerLink
Log in

A novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from Alphaproteobacterium strain Q-1

  • Biotechnologically relevant enzymes and proteins
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Alphaproteobacterium strain Q-1 produces an extracellular multicopper oxidase (IOX), which catalyzes iodide (I–) oxidation to form molecular iodine (I2). In this study, the antimicrobial activity of the IOX/iodide system was determined. Both Gram-positive and Gram-negative bacteria tested were killed completely within 5 min by 50 mU mL–1 of IOX and 10 mM iodide. The sporicidal activity of the system was also tested and compared with a common iodophor, povidone-iodine (PVP-I). IOX (300 mU mL–1) killed Bacillus cereus, B. subtilis, and Geobacillus stearothermophilus spores with decimal reduction times of 2.58, 7.62, and 40.9 min, respectively. However, 0.1 % PVP-I killed these spores with much longer decimal reduction times of 5.46, 38.0, and 260 min, respectively. To evaluate the more superior sporicidal activity of the IOX system over PVP-I, the amount of free iodine (non-complexed I2) was determined by an equilibrium dialysis technique. The IOX system included more than 40 mg L–1 of free iodine, while PVP-I included at most 25 mg L–1 free iodine. Our results suggest that the new enzyme-based antimicrobial system is effective against a wide variety of microorganisms and bacterial spores, and that its strong biocidal activity is due to its high free iodine content, which is probably maintained by re-oxidation of iodide released after oxidation of cell components by I2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Amachi S, Muramatsu Y, Akiyama Y, Miyazaki K, Yoshiki S, Hanada S, Kamagata Y, Ban-nai T, Shinoyama H, Fujii T (2005) Isolation of iodide-oxidizing bacteria from iodide-rich natural gas brines and seawaters. Microb Ecol 49:547–557

    Article  CAS  PubMed  Google Scholar 

  • Atemnkeng MA, Plaizier-Vercammen J, Schuermans A (2006) Comparison of free and bound iodine and iodide species as a function of the dilution of three commercial povidone-iodine formulations and their microbiocidal activity. Int J Pharm 317:161–166

    Article  CAS  PubMed  Google Scholar 

  • Cooper RA (2007) Iodine revisited. Int Wound J 4:124–137

    Article  PubMed  Google Scholar 

  • Ehara A, Suzuki H, Kanesaki Y, Yoshikawa H, Amachi S (2014) Draft genome sequence of strain Q-1, an iodide-oxidizing Alphaproteobacterium isolated from natural gas brine water. Genome Announc 2:e00659–14

    Article  PubMed Central  PubMed  Google Scholar 

  • Fuglsang CC, Johansen C, Christgau S, Adler-Nissen J (1995) Antimicrobial enzymes: application and future potential in the food industry. Trends Food Sci Technol 6:390–396

    Article  CAS  Google Scholar 

  • Gottardi W (1983) Chapter 8: iodine and iodine compounds. In: Block SS (ed) Disinfection, sterilization and preservation, 3rd edn. Lea & Febiger, Philadelphia, pp 183–196

    Google Scholar 

  • Hansen EH, Albertsen L, Schäfer T, Johansen C, Frisvad JC, Molin S, Gram L (2003) Curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant. Appl Environ Microbiol 69:4611–4617

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hickey J, Panicucci R, Duan Y, Dinehart K, Murphy J, Kessler J, Gottardi W (1997) Control of the amount of free molecular iodine in iodine germicides. J Pharm Pharmacol 49:1195–1199

    Article  CAS  PubMed  Google Scholar 

  • Muramatsu Y, Fehn U, Yoshida S (2001) Recycling of iodine in fore-arc areas: evidence from the iodine brines in Chiba, Japan. Earth Planet Sci Lett 192:583–593

    Article  CAS  Google Scholar 

  • Sakuma A (2005) Manufacturing, recovery and recycling of iodine. In: Yokoyama M (ed) Function & application of iodine compounds. CMC Publishing, Tokyo, pp 15–27 (in Japanese)

    Google Scholar 

  • Suzuki M, Eda Y, Ohsawa S, Kanesaki Y, Yoshikawa H, Tanaka K, Muramatsu Y, Yoshikawa J, Sato I, Fujii T, Amachi S (2012) Iodide oxidation by a novel multicopper oxidase from Alphaproteobacterium strain Q-1. Appl Environ Microbiol 78:3941–3949

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Takikawa K, Nakano M, Arita T (1978) Change in apparent permeability of iodine in the presence of polyvinylpyrrolidone. Chem Pharm Bull 26:874–879

    Article  CAS  Google Scholar 

Download references

Funding

This work was partially supported by grants from the Japan Foundation for Applied Enzymology, JSPS KAKENHI (20780049), and the Institute of Fermentation, Osaka, Japan, to S. A.

Conflict of interest

The authors confirm that they have no conflict of interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Author information

Authors and Affiliations

  1. Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba, 271-8510, Japan

    Tri Yuliana, Kyota Ebihara, Mio Suzuki, Chie Shimonaka & Seigo Amachi

  2. Food Technology Department, Padjadjaran University, Jalan Raya Bandung Sumedang KM21, Bandung, 45363, Indonesia

    Tri Yuliana

Authors
  1. Tri Yuliana
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyota Ebihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mio Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chie Shimonaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seigo Amachi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Seigo Amachi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 172 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yuliana, T., Ebihara, K., Suzuki, M. et al. A novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from Alphaproteobacterium strain Q-1. Appl Microbiol Biotechnol 99, 10011–10018 (2015). https://doi.org/10.1007/s00253-015-6862-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-015-6862-0

Keywords

Search

Navigation