Abstract
Shewanella colwelliana, a marine bacterium isolated in association with the oyster Crassostrea virginica, produces an abundant exopolysaccharide with potential commercial value as an adhesive under aqueous conditions. Its utilization of glucose was modulated by stoichiometric concentrations of yeast extract. In Brain Heart Infusion medium containing glucose, growth was diauxic with delayed glucose utilization and incorporation into exopolysaccharide. Data from radio-respirometry protocols indicate that glucose is catabolized through a combination of the hexose monophosphate and Entner-Doudoroff pathways. Exopolysaccharide production could be significantly enhanced by adjusting glucose concentrations of the growth medium.
Similar content being viewed by others
References
AbuG.O., WeinerR.M., BonarD.B. & ColwellR.R. 1986 Extracellular polysaccharide production by a marine bacterium. Biodeterioration 6, 543–549.
AbuG.O., WeinerR.M., RiceJ. & ColwellR.R. 1991 Properties of an extracellular adhesive polymer from the marine bacterium, Shewanella colwelliana. Biofouling 3, 69–84.
AradS., AddaM. & CohenE. 1985 The potential of production of sulfated polysaccharides from Porphyridium. In Biosalinity in Action: Bioproduction with Saline Water, eds PasternakD. & San PietroA. pp. 117–127. Amsterdam: Marinus Nijhoff.
AustinB. (ed) 1988 Marine Microbiology. Cambridge: Cambridge University Press.
BruinenbergP.M., VanDijkenJ.P. & ScheffersW.A. 1986 A radiorespirometric study on the contribution of the hexose monophosphate pathway in glucose metabolism in Candida utilis CBS 621 grown in glucose-limited chemostat cultures. Journal of General Microbiology 132, 221–229.
CorpeW.A. 1970 An acidic polysaccharide produced by a primary film-forming marine bacterium. Developments in Industrial Microbiology 11, 402–412.
DawesE.A. (ed) 1986 Microbial Energetics. New York: Blackie, Chapman and Hall.
DobrogoszW.J. 1981 Enzymatic activity. In Manual of Methods of General Bacteriology, eds GerhardtP., MurrayR.G.E., CostilowR.N., NesterE.W., WoodW.A., KriegN.R. & PhillipsG.B. pp. 365–404. Washington DC: American Society of Microbiology.
GeeseyG.G. 1982 Microbial exopolymers: ecology and economic considerations. ASM News 4, 9–14.
JarmanT.R. & PaceG.W. 1984 Energy requirements for microbial exopolysaccharide synthesis. Archives of Microbiology 137, 231–235.
LabareM.P., GuthrieK. & WeinerR.M. 1989 Polysaccharide exopolymer adhesives from periphytic marine bacteria. Journal of Adhesion Science Technology 3, 213–223.
LemosM.L., ToranzoA.E. and BarjaJ.L. 1985 Modified medium for the oxidation-fermentation test in the identification of marine bacteria. Applied and Environmental Microbiology 49, 1541–1543.
NorbergA.B. & EnforsS. 1982 Production of extracellular polysaccharide by Zooglea ramigera. Applied and Environmental Microbiology 44, 1231–1237.
OkamiY. 1986 Marine microorganisms as a source of bioactive agents. Microbial Ecology 12, 65–78.
SandfordP.A. 1979 A survey of possible new polysaccharides. In Polysaccharides in Food, eds BlanchfordJ.M.V. & MitchellJ.K. pp. 251–262. London: Butterworths.
SandfordP.A. 1985 Applications of marine polysaccharides in the chemical industry. In Biotechnology in the Marine Sciences: Biotechnology of Marine Polysaccharides, eds ColwellR.R., PariserE.R. & SinskeyA.J. pp. 451–516. New York: McGraw-Hill.
SledjeskiD. & WeinerR. 1993 Production and characterization of monoclonal antibodies specific for Shewanella colwelliana exopolysaccharides. Applied and Environmental Microbiology 59, 1565–1572.
SutherlandI.W. 1983 Microbial exopolysaccharides and their role in microbial adhesion in aqueous systems. CRC, Critical Reviews in Microbiology 10, 173–201.
WangC.H. 1963 Metabolism studies by radiorespirometry. In Advances in Tracer Methodology, Vol 1, ed RothchildS. pp. 274–290. New York: Plenum.
WangC.H. 1972 Radiorespirometric methods. Methods in Microbiology 6B, 185–230.
WangC.H., SternI., GilmourC.M., KlungsoyrS., ReedD.J., BiallyJ.J., ChristensenB.E. & CheldelinV.H. 1958 Comparative study of glucose metabolism by the radiorespirometric method. Journal of Bacteriology 76, 207–216.
WeinerR.M. & ColwellR.R. 1982 Induction of Settlement and Metamorphosis in Crassostrea virginica by a Melanin Synthesizing Bacterium. Sea Grant Technical Report UM.SG.TS.82-05. College Park, MD: University of Maryland.
WeinerR.M., WalchM., LabareM.P., BonarD.B. & ColwellR.R. 1989 Effect of biofilms of the marine bacterium Alteromonas colwelliana (LST) on the set of the oysters Crassostrea gigas (Thunberg, 1793) and C. virginica (Gmelin, 1791). Journal of Shellfish Research 8, 117–123.
Additional information
G.O. Abu was and R. Weiner and R.R. Colwell are with the Department of Microbiology, University of Maryland, College Park, MD 20742, USA. G. O. Abu is now with the Department of Microbiology, University of Port Harcourt, P.M.B. 5323, PH, Nigeria.
Rights and permissions
About this article
Cite this article
Abu, G.O., Weiner, R. & Colwell, R.R. Glucose metabolism and polysaccharide accumulation in the marine bacterium, Shewanella colwelliana . World J Microbiol Biotechnol 10, 543–546 (1994). https://doi.org/10.1007/BF00367663
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00367663