Abstract
We have established a novel cell line from a Vero cell derivative that is useful for expression of exogenous genes and protein production. Parental Vero-317 cells can grow in biotin-containing Eagle's MEM without supplements. By transforming this cell line with replication origin-defective SV40 DNA, which contains a temperature-sensitive tsA58 large T antigen gene, we established the Verots S3 cell line that amplified a SV40-origin containing plasmid. The cell line expressed a human growth hormone (hGH) gene insert with higher efficiency than COS-7 cells in 5% serum-containing MEM and could grow and continue hGH expression in protein-free MEM. However, temperature-sensitive shut down of hGH production was observed not immediately but 3 days after the temperature shift from 33°C to 39.5°C.
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Ohno, T., Wang, X., Kurashima, J. et al. A novel Vero cell line for use as a mammalian host-vector system in serum-free medium. Cytotechnology 7, 165–172 (1991). https://doi.org/10.1007/BF00365927
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DOI: https://doi.org/10.1007/BF00365927