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Chloroplast DNA differences in the genus Oenothera subsection Munzia: a short direct repeat resembling the lambda chromosomal attachment site occurs as a deletion/insertion within an intron of an NADH-dehydrogenase gene

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Summary

A small restriction fragment length mutation has been mapped in the large inverted repeats of the chloroplast (cp) DNA of Munzia-Oenothera species (vom Stein and Hachtel 1986). This mutation could be localized within the intron of a reading frame presumably coding for subunit B of an NADH-dehydrogenase (ndhB). Sequence analysis revealed a 24 by duplication/deletion. The predicted secondary structure of the ndhB-intron is altered by this duplication/deletion. Part of the directly repeated segment shows remarkable similarity to the phage lambda attachment site. Evidence is presented for similar sequences in other plastome regions where deletions/insertions have been found. Furthermore, the locations of the genes for other components of the NADH-dehydrogenase (ndhA, ndhC, ndhD, ndhE, ndhF) were established by heterologous hybridization using gene probes from tobacco cpDNA.

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Abbreviations

bp :

base pairs

kB :

1,000 bp

cpDNA :

chloroplast DNA

Oe :

Oenothera

att-lambda :

attachment site of bacterio-lambda

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Authors and Affiliations

  1. Botanisches Institut der Universität Bonn, Kirschallee 1, D-5300, Bonn 1, Germany

    J. vom Stein & W. Hachtel

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  1. J. vom Stein
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  2. W. Hachtel
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vom Stein, J., Hachtel, W. Chloroplast DNA differences in the genus Oenothera subsection Munzia: a short direct repeat resembling the lambda chromosomal attachment site occurs as a deletion/insertion within an intron of an NADH-dehydrogenase gene. Curr Genet 13, 191–197 (1988). https://doi.org/10.1007/BF00365654

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  • DOI: https://doi.org/10.1007/BF00365654

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