Summary
A strain of fission yeast carrying replicating instability (RI) will segregate mitotically three types of cells: unstable (still RI-carrying) cells, stable identical mutants and stable non-mutants. RI in fission yeast has previously been considered as a specific type of premutational lesion capable of (1) being replicated as such and (2) reverting at an appreciable rate to the normal state as well as changing into a stable mutation. In the present work genetic analysis of a previously studied RI-carrying strain showed this strain to be a diploid, most probably heterozygous for a recessive mutation. It was possible to construct other unstable heterozygous strains segregating predetermined mutants but not to induce RI by UV-irradiation of haploid cells. Thus evidence is presented against the premutational nature of RI in fission yeast.
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Kurennaya, O.N., Devin, A.B. On the nature and specificity of replicating instability in fission yeast. Curr Genet 10, 291–296 (1985). https://doi.org/10.1007/BF00365625
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DOI: https://doi.org/10.1007/BF00365625