Abstract
Methods were developed for the culture of cells derived from tissues of the sea lamprey (Petromyzon marinus). Cultures were initiated from gill, liver, muscle and gut from larvae and newly transformed individuals and brain, heart, kidney and ovary from sexually mature adults. The lamprey cells were viable for up to six months in culture and cells from ovary, muscle, gut, gill and liver were propagated for multiple passages. For all cultures except liver, optimal cell attachment and spreading was obtained on surfaces coated with fibronectin and collagen. Optimal liver cell attachment was achieved on basement membrane. Cells synthesizing DNA were detected by precursor incorporation in five week-old cultures derived from adult and larval tissues. Metabolic labeling experiments with [35S]-methionine demonstrated that cultures initiated from liver and ovary continued to synthesize and release proteins into the medium for several weeks. Ultrastructural examination revealed the presence of ciliated cells in cultures from brain and the accumulation of lipid in epithelial cells derived from liver and gill.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergstedt RA and Seelye JG (1992) Current and proposed alternative methods for the control of sea lampreys. Publication of the U.S. Fish and Wildlife Service, National Fisheries Research Center, Hammond Bay Biological Station, Millerburg, MI.
Bodznick D and Northcutt RG (1981) Electroreception in lampreys: Evidence that the earliest vertebrates were electroreceptive. Science 212: 465–467.
Bolliet V, Ali MA, Anctil M and Zachmann A (1993) Melatonin secretion in vitro from the pineal complex of the lamprey Petromyzon marinus. Gen. Comp. Endocrinol. 89: 101–106.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Colbert EH and Morales M (1991) Evolution of the vertebrates. Wiley-Liss, New York, pp 18–25.
Collodi P and Barnes DW (1990) Mitogenic activity from trout embryos. Proc. Natl. Acad. Sci., USA. 87: 3498–3502.
Collodi P, Kamei Y, Ernst T, Miranda C, Buhler DR and Barnes DW (1992) Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell Biol. Toxicol. 8: 43–60.
Davis GR, Troxel MT, Kohler VJ, Grossmann EM and McClellan AD (1993) Time course of locomotor recovery and functional regeneration in spinal-transected lamprey: kinematics and electromyography. Exp. Brain Res. 97: 83–95.
Ghosh C and Collodi P (1994) Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos. Cytotechnology 14: 21–26.
Hagevik A and McClellan AD (1994) Role of excitatory amino acids in brainstem activation of spinal locomotor networks in larval lamprey. Brain Res. 636: 147–152.
Hanson LH and Manion PJ (1980) Sterility method of pest control and its potential role in an integrated sea lamprey (Petromyzon marinus) control program. Can. J. Fish. Aquat. Sci. 37: 2108–2117.
Hardisty MW and Potter IC (1971) The behavior, ecology, and growth of larval lampreys, in Hardisty MW and Potter IC (eds.) The Biology of Lampreys vol. 1 (85–125) Academic Press, London.
Howell JH, Lech JJ and Allen JL (1980) Development of sea lamprey (Petromyzon marinus) larvicides. Can. J. Fish. Aquat. Sci. 37: 2103–2107.
Knox CJ, Boyd SK and Sower SA (1994) Characterization and localization of gonadotropin-releasing hormone receptors in the adult female sea lamprey, Petromyzon marinus. Endocrinology 134: 492–498.
Langille RM and Hall BK (1993) Calcification of cartilage from the lamprey Petromyzon marinus (L.) in vitro. Acta Zoologica 74: 31–41.
Li W, Sorensen PW, Gallaher DD (1995) The olfactory system of migratory adult sea lamprey (Petromyzon marinus) is specifically and acutely sensitive to unique bile acids released by conspecific larvae. J. Gen. Physiol. 105: 569–587.
Lurie DE, Pijak DS and Selzer ME (1994) Structure of reticulospinal axon growth cones and their cellular environment during regeneration in the lamprey spinal cord. J. Comp. Neurol. 344: 559–580.
McClellan AD (1994) Time course of locomoter recovery and functional regeneration in spinal cord-transected lamprey: In vitro preparations. J. Neurophysiol 72: 847–860.
Mestres P and Rascher K (1994) The ventricular system of the pigeon brain: A scanning electron microscope study. J. Anatomy 184: 35–58.
Nonaka M (1994) Molecular analysis of the lamprey complement system. Fish Shellfish Immunol. 4: 437–446.
Sower SA, Chiang YC, Lovas S and Conlon JM (1993) Primary structure and biological activity of a third gonadotropin-releasing hormone from lamprey brain. Endocrinology 132: 1125–1131.
Teeter J (1980) Pheromone communication in sea lampreys (Petromyzon marinus): Implications for population management. Can. J. Fish. Aquat. Sci. 37: 2123–2132.
Wallen P, Shupliakov O and Hill RH (1993) Origin of phasic synaptic inhibition in myotomal motoneurons during fictive locomotion in the lamprey. Exp. Brain Res. 96: 194–202.
Vigh B and Teichmann I (1993) Development of the photoreceptor outer segment-like cilia of the CSF-contacting pinealocytes of the ferret (Putorius furo). Arch. Hist. Cytol. 56: 485–493.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ma, C., Collodi, P. Culture of cells from tissues of adult and larval sea lamprey. Cytotechnology 21, 195–203 (1996). https://doi.org/10.1007/BF00365342
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00365342