Skip to main content

Advertisement

SpringerLink
Log in

A new parental cell line for human x human hybridoma production

  • Technical Note
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The potential of a new HAT-sensitive human lymphoblastoid cell line TK6 TGr.P1. as a fusion partner was assessed, by comparison with the established human parental cell line UC729.6. Both of these cell lines were fused with the peripheral blood mononuclear cells of a patient with B-chronic lymphocytic leukaemia. The hybridomas generated in these fusion experiments were analysed by the fluorescence activated cell sorter and karyotyping. An anti-idiotype ELISA assay detected the presence of the patient's characteristic idiotype bearing immunoglobulin in the supernatant of a number of the hybridoma cell lines generated in both fusions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cole J, Arlett CF and Muriel WJ (1985) The absence of mutagenic activity of benzidine and 4,4 — diaminsterphenyl in TK6 human lymphoblast cells. In: Parry JM and Arlett CF Comparative Genetic Toxicology, Chapter 35, pp 301–306, MacMillan.

  2. Eisenhut M and Chone B (1979) Studies of the binding of ethidium bromide to cells before and after enzyme treatment. Histochemistry 62: 207.

    Google Scholar 

  3. Glassey MC, Handley HH, Hagiwara H and Royston I (1983) UC729.6, a human lymphoblastoid B-cell line useful for generating antibody secreting human-human hybridomas. Proceedings of the National Academy of Sciences USA 80: 6327.

    Google Scholar 

  4. Millar RA, Maloney DG, Warnke R and Levy R (1982) Treatment of B-cell lymphoma with monoclonal anti-idiotype antibody. New England Journal of Medicine 306: 517.

    Google Scholar 

  5. Ruddle FH and Cregan RP (1975) Parasexual approaches to genetics of man. Annual Reviews in Genetics 9: 407.

    Google Scholar 

  6. Seabright M (1971) A rapid banding technique for human chromosomes. Lancet ii: 971.

    Google Scholar 

  7. Stevenson GT and Glennie MJ (1985) Surface immunoglobulin of B-lymphocytic tumours as a therapeutic target. Cancer Surveys 4 (No. 1): 213.

    Google Scholar 

  8. Thilly WG, DeLuca JG, Surth EE, Hotte H, Caden DA, Krolewski JJ, Liber HL, Skopek TR, Slapikoff SA, Pizard RJ and Penman BW (1980) Gene Locus Mutant assays in diploid human lymphoblast lines.

  9. Serres FJ and Hollaender A. Chemical Mutagens, Volume 6, Chapter 12, pp 331–364. Plenum Publishing Corporation.

Download references

Author information

Authors and Affiliations

  1. Division of Biologics, Centre for Applied Microbiology, PHLS, Porton Down, Wiltshire, United Kingdom

    S. A. Clark

  2. M.R.C. Cell Mutation Unit, University of Sussex, Falmer, Brighton, United Kingdom

    S. E. James

  3. Wessex Regional Cytogenetics Unit, General Hospital, Salisbury, Wiltshire, United Kingdom

    M. Fitchett

Authors
  1. S. A. Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. E. James
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Fitchett
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Clark, S.A., James, S.E. & Fitchett, M. A new parental cell line for human x human hybridoma production. Cytotechnology 1, 359–363 (1988). https://doi.org/10.1007/BF00365082

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00365082

Key words

Search

Navigation