Skip to main content
Log in

Polystyrene substratum for bulk culture of anchorage dependent cells

  • Reviews
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Twisted ribbons made of polystyrene were used as a packing material for the cultivation of anchorage dependent cells. Normal human fibroblast cells grown on this support in a laboratory scale reactor reached densities of about 5–7×105 cells/ml. The cells adhered strongly to the carrier and no cell detachment was observed upon transfer to serum free medium. The properties of this packing material and its potential use are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Curtis ASG, Forrester JV, McInnes C and Lawrie F (1983) Adhesion of cells to polystyrene surfaces. J. Cell Biol. 97: 1500–1506.

    Google Scholar 

  2. Douglas WHJ, McAtler JA, Dellorco RJ and Phelps D (1980) Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix. In vitro 16: 306–312.

    Google Scholar 

  3. House W, Shearer M and Maroudas NG (1972) Method for bulk culture of animal cells on plastic film. Exp. Cell Res. 71: 293–296.

    Google Scholar 

  4. Kadouri A, Bohak Z, Maroudas NG and Sussman MV (1987) Packing bed materials for the growth of anchorage dependent cells. The 8th Meeting of ESACT on modern approaches to animal cell technology. Tiberias, Israel April 1987.

  5. Knazek RA, Gullino PM, Kohler PO and Dedrick RL (1972) Cell culture on artificial capillaries: an approach to tissue growth in vitro. Science 178: 65–67.

    Google Scholar 

  6. Lyderson BK, Pugh GG, Duncan EC, Overman KT, Johanson DM and Sharma BP (1983) A novel ceramin material for large scale animal cell cultures. 34th Annual Meeting of the Tissue Culture Association. Orlando, Fl. June 1983.

  7. Maroudas NG (1974) Monolayer mass culture on disposable plastic spirals. Meth. Cell Biol. 8: 93–99.

    Google Scholar 

  8. Maroudas NG (1978) Sulphonated polystyrene as an optimal substratum for the adhesion of mesenchymal cells in monovalent and divalent saline solutions. J. Cell Physiology 100: 110–120.

    Google Scholar 

  9. McKeehan WL and Ham RG (1976) Stimulation of clonal growth of normal fibroblasts with substrate coated with basic polymers. J. Cell Biol. 71: 727–734.

    Google Scholar 

  10. Santero GG (1972) The ‘Rotary Column’ Method for growth of large-scale quantities of cell monolayers. Biotechnol. Bioeng. 14: 753–775.

    Google Scholar 

  11. Spier RE and Whiteside JP (1976) The production of foot and mouth disease virus from BHK 21C13 cells grown on the surface of glass spheres. Biotechnol. Bioeng. 18: 649–657.

    Google Scholar 

  12. Spier RW, Whiteside JP and Bolt K (1977) Trypsinization of BHK 21 monolayer cells grown in two large-scale unit process systems. Biotechnol. Bioeng. 19: 1735–1738.

    Google Scholar 

  13. Van Wezel AL (1967) Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature (Lond) 216: 64–65.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kadouri, A., Sher, D. & Maroudas, N.G. Polystyrene substratum for bulk culture of anchorage dependent cells. Cytotechnology 1, 301–307 (1988). https://doi.org/10.1007/BF00365075

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00365075

Key words

Navigation