Abstract
Addition of a 3 M KCl extract of a human lung adenocarcinoma PC-8 cells to the culture media of lymphocytes, which were isolated from normal donors and from lung or breast cancer patients, elevated immunoglobulin (Ig) production by 3–5 times in the presence of pokeweed mitogen. However, addition of higher concentration of the extract inhibited Ig production and proliferation of lymphocytes. The Ig production stimulating factor (IPSF) was separated from the inhibiting factor, using 50% ammonium sulfate precipitation. Upon chromatofocusing, IPSF activity was detected mainly in the pH 4.5 fraction but minor activity was also detected in other pH fractions. IPSF also enhanced Ig production of a B-lymphoblastoid cell line transformed by Epstein-Barr virus and a human-human hybridoma, by more than 2-fold. This suggests that IPSF interacts with B-lymphocytes directly to enhance their Ig production. IPSF activity was also detected diversely in human lung squamous carcinoma QG56, human B-lymphoblastoid HO-323, and a T cell line CEM.
Similar content being viewed by others
References
Engvall E and Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry 8: 871–874.
Herberman RB (1974) Cell-mediated immunity to tumor cells. Advan. Cancer Res. 19: 207–263.
Lachmann PJ and Mitchison NA (1982) Immune response to tumors. In: Clinical Aspects of Immunology, 4th ed. Lachmann P and Peters K (ed.), pp 1263–1278, Blackwell, Oxford.
Maeda-Takekoshi F, Takekoshi M, Tanaka S, Watanabe Y, Dosako S and Kikuchi E (1984) Transformation of human colostrum lymphocytes with Epstein-Barr virus and the production of IgA in culture. Tokai J. Exp. Clin. Med. 9: 103–108.
Mosmann T (1983) Rapid colorimetic assay for cellular growth and survival: Application to proliferation and cytotoxity assays. J. Immunol. Methods 65: 55–63.
Makherji B, Wihelm SA, Guha A and Ergin MT (1986) Regulation of cellular immune response against autologous human melanoma. I. Evidence for cell-mediated suppression of in vitro cytotoxic immune response. J. Immunol. 136: 1888–1892.
Murakami H, Hashizume S, Ohashi H, Shinohara K, Yasumoto K, Nomoto K and Omura H (1985) Human-human hybridomas secreting antibodies specific to human lung carcinoma. In Vitro Cell. Develop. Biol. 21: 593–596.
Naor D (1980) Suppressor cells: permitters and promoters of malignancy? Adv. Cancer Res. 29: 45–125.
Ohashi H, Hashizume S, Murakami H, Aihara K, Shinohara K and Omura H (1986) HO-323, a human B-lymphoblastoid cell line useful for making human-human hybridomas. Cell Biol. Int. Rep. 10: 77–83.
Shinmoto H, Murakami H, Dosako S, Shinohara K and Omura H (1986) Human-human hybridomas secreting IgM-liker immunoglobulin having α and μ heavy chains. Agric. Biol. Chem. 50: 2217–2223.
Stern AC, ERb P and Gisler RH (1979) Ia-bearing bone marrow-cultured macrophages induce antigen-specific helper T cells for antibody synthesis. J. Immunol. 123: 612–615.
Vose BM and Klein E (1977) Human tumour-lymphocytes interaction in vitro. V. Comparison of the reactivity of tumour-infiltrating, blood and lymph-node lymphocytes with autologous tumour cells. Int. J. Cancer 20: 895–902.
Vose BM and White W (1983) Tumour-reactive lymphocytes stimulated in mixed lymphocytes and tumor culture. Cancer Immunol. Immunother. 15: 227–236.
Waxdal MJ (1974) Isolation, characterization, and biological activities of five mitogens from pokeweed. Biochemistry 13: 3671–3677.
Werkmeister J, Zbroja R, McCarthy W and Horsey P (1980) Detection of an inhibitor of cell division in cultures of tumour cells with immunosuppressive activity in vitro. Clin. Exp. Immunol. 40: 168–177.
Yokoyama K, Yano O, Terano T and Osawa T (1976) Purification and biological activities of pokeweed (Phytolacca americana) mitogens. Biochem. Biophys. Acta 427: 443–452.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shinmoto, H., Murakami, H., Yamada, K. et al. Immunoglobulin production stimulating and inhibiting factors derived from human lung adenocarcinoma PC-8 cells. Cytotechnology 1, 295–300 (1988). https://doi.org/10.1007/BF00365074
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00365074