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Dexamethasone regulation of terminal differentiation in 3T3-F442A preadipocyte cell line

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Abstract

The 3T3-F442A preadipocyte cell line was previously shown to possess specific glucocorticoid receptors whose number increased in the time course of differentiation. We have examined the effects of a three day dexamethasone treatment, added at confluence, on cells differentiated in the presence or absence of insulin. Triglyceride accumulation, polyamine content as well as glycerophosphate dehydrogenase and fatty acid synthetase activities were measured during the adipose conversion. We have also determined 2-deoxyglucose uptake in non-differentiated and differentiated cells. Dexamethasone was shown to decrease the adipose conversion by 3T3-F442A cells in the presence or absence of insulin. Intracellular spermidine content in differentiating cells was sensitive to dexamethasone and insulin in the same way as an enzymatic marker of terminal differentiation, glycerophosphate dehydrogenase. Dexamethasone decreases the 2 deoxyglucose uptake in non-differentiated and differentiated cells while insulin increases this uptake only in differentiated cells. This work shows that glucocorticoids inhibit adipocyte metabolism at distinct levels and suggests that these hormones might play an important role in the regulation of adipose tissue mass.

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Abbreviations

DEX:

dexamethasone

FAS:

fatty acid synthetase

GPDH:

glycerophosphate dehydrogenase

MIX:

1-methyl-3-isobutylxanthine

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Authors and Affiliations

  1. Institut Biomédical des Cordeliers, INSERM U177, 15, rue de l'école de médecine, 75006, Paris, France

    Naïma Moustaïd, Bernard Hainque & Annie Quignard-Boulangé

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  1. Naïma Moustaïd
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  2. Bernard Hainque
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  3. Annie Quignard-Boulangé
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Moustaïd, N., Hainque, B. & Quignard-Boulangé, A. Dexamethasone regulation of terminal differentiation in 3T3-F442A preadipocyte cell line. Cytotechnology 1, 285–293 (1988). https://doi.org/10.1007/BF00365073

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  • DOI: https://doi.org/10.1007/BF00365073

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