Abstract
We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H 1 restriction fragment present in the λ12a DNA clone to the D-TL region of H-2 p. Only a single 8.8 kb Barn H1 fragment can be assigned to K p by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of λ12a is presented.
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Macchi, M.J., Woodward, J.G., McLaughlin-Taylor, E. et al. Cloning and identification of the H-2D p gene. Immunogenetics 19, 195–204 (1984). https://doi.org/10.1007/BF00364763
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DOI: https://doi.org/10.1007/BF00364763