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CML characterization of a product of a second class I locus in the rat MHC

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Abstract

In the rat, genes that control the expression of target antigens detected by cell-mediated lympholysis (CML) are present in the major histocompatibility complex (MHC). The relationship of these loci, CT and Ag-L, to each other and to other loci within the MHC is unknown. In this report, we demonstrate the existence of a CML target antigen in the (DA × BN)F1 anti-DA.11(BI) strain combination. The gene coding for this antigen is linked to the RT1 complex as indicated by the CML reactivity of targets from backcross and congenic animals. Inhibition studies demonstrated that this antigen has the widespread tissue distribution characteristic of class I antigens, and the gene coding for this CML antigen maps coincident with the RT1.E class I locus as indicated by the lysis of targets from the recombinant strains r10 and r11. The CML can be blocked by antisera directed against a product of the RT1.E locus. The locus controlling this CML reactivity, like CT and Ag-L, has been separated from RT1.A by recombination; unlike CT and Ag-L, the product of this CML locus appears to be identical with an RT1.E allelic product that has been serologically identified and biochemically characterized.

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Abbreviations

MHC:

major histocompatibility complex

CML:

cell-mediated lympholysis

Con A:

concanavalin A

SD:

standard deviation

HEPES:

N-2-hydroxy-piperazine-N′-2-ethanesulfonic acid

CPM:

counts per minute

grc :

growth and reproduction complex

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Authors and Affiliations

  1. Department of Pathology, University of Pittsburgh School of Medicine, Room 712 Scaife Hall, 15261, Pittsburgh, Pennsylvania

    Monica Liebert, Heinz W. Kunz, Thomas J. Gill III & Donald V. Cramer

Authors
  1. Monica Liebert
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  2. Heinz W. Kunz
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  3. Thomas J. Gill III
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  4. Donald V. Cramer
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Liebert, M., Kunz, H.W., Gill, T.J. et al. CML characterization of a product of a second class I locus in the rat MHC. Immunogenetics 16, 143–155 (1982). https://doi.org/10.1007/BF00364401

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  • DOI: https://doi.org/10.1007/BF00364401

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