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Rabbit heavy chain haplotypes — Allotypic determinants expressed byVH-CH recombinants

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Abstract

This report summarizes our current understanding of the heavy chain haplotypes found in our laboratories' rabbits. Independently derived data from several laboratories have been synthesized into a consistent picture of the linked inheritance of allotypic markers found on the different heavy chain classes and subclasses of rabbit immunoglobulins in pedigreed rabbits, including the families of three apparentVH-CH recombinants. In one recombinant, the entire group ofCH markers (Cμ, Cγ, and Cα) recombined with the set ofVH. Although in the other two recombinants all CH markers may also have recombined as a group, in one of these only IgG and IgACH genes were informative; in the other recombinant, only the IgG allotypes were informative. Some allotypic determinants found on IgM molecules (“conformational”) appear only when a specific variable region allotype (VHa) is combined with a specific μ constant region allotype (Cμ). New combinations ofVHa and Cμ allotypes were generated in two of the genetic recombinants and led to new “conformational” determinants. The gains and losses observed lend support to the hypothesis that the determinants result from conformations generated by the combination of allotype-specificVH and Cμ protein sequences. Conceivably, DNA events that joinVH to diversity (D)- and joining (J)-coding sequences or mRNA processing events that splice J to Cμ could be involved in generating the sequences that form allotype-specific determinants.

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References

  • Avrameas, S. and Ternynck, T.: Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J. Biol. Chem. 242: 1651–1659, 1967

    Google Scholar 

  • Carta-Sorcini, M., Appella, E., Inman, J. K., and Mage, R.: The immunoglobulins derived from aVH-CH recombinant rabbit and its normal relatives. II. Chemical studies of allotype related differences in Fd and Fc fragments. Immunochemistry 10: 449–453, 1973

    Google Scholar 

  • Dray, S., Young, G. O., and Gerald, L.: Immunochemical identification and genetics of rabbit γ-globulin allotypes. J. Immunol. 91: 403–415, 1963

    Google Scholar 

  • Dray, S., Kim, B. S., and Gilman-Sachs, A.: Allogroups of rabbit Ig heavy chains. Ann. Immunol. (Paris) 125: 41, 1974

    Google Scholar 

  • Dubiski, S.: Immunochemistry and genetics of a “new” allotypic specificity Ae14 of rabbit γG immunoglobulins: Recombination in somatic cells. J. Immunol. 103: 120–128, 1969

    Google Scholar 

  • Eby, W. C., Kim, B. S., Dray, S., Young-Cooper, G. O., and Mage, R. G.: Detection of the e14 and e15 rabbit allotypic specificities by immunodiffusion in PEG agar. Immunochemistry 10: 417–418, 1973

    Google Scholar 

  • Gilman-Sachs, A. and Dray, S.: Identification and genetic control of two rabbit IgM allotypic specificities. Eur. J. Immunol. 2: 505–509, 1972

    Google Scholar 

  • Gilman-Sachs, A., and Dray, S.: Identification and genetic control of the n83 and n84 allotypes of rabbit IgM. J. Immunol. 118: 1580–1585, 1977

    Google Scholar 

  • Hamers-Casterman, C. and Hamers, R.: A second crossing-over between d and a locus in rabbit immunoglobulin γ chain. Immunogenetics 2: 597–603, 1975

    Google Scholar 

  • Hanly, W. C. and Gilman-Sachs, A.: Immunoglobulin allotypes: Rabbit. Allogroups of the Ig heavy-chain chromosomal region: Rabbit. In P. L. Altman and D. D. Katz (eds.): Inbred and Genetically Defined Strains ofLaboratory Animals, Part 2, pp. 592–606, FASEB Biological Handbook, Bethesda, Maryland, 1979

  • Horng, W. J., Roux, K. H., Gilman-Sachs, A., and Dray, S.: An increase in the number of recombinant molecules and other effects of the simultaneous allotype suppression of trans-chromosomal a VH and n Cμ Ig gene products. Mol. Immunol., in press, 1981

  • Hunter, W.: Radioimmunoassay. In D. M. Weir (ed.): Handbook of Experimental Immunology 1, 2nd edit., Chapter 17, Blackwell Scientific Publications, Oxford, 1973

    Google Scholar 

  • Kindt, T. J., Mandy, W. J., and Todd, C. W.: The action of cyanogen bromide on rabbit IgG molecules of allotypes A11 and A12. Immunochemistry 7: 467–477, 1970

    Google Scholar 

  • Kindt, T. J. and Mandy, W. J.: Recombination of genes coding for constant and variable regions of immunoglobulin heavy chains. J. Immunol. 108: 1110–1113, 1972

    Google Scholar 

  • Knight, K. L., Malek, T. R., and Hanly, W. C.: Recombinant rabbit secretory immunoglobulin molecules: Alpha chains with maternal (paternal) variable-region allotypes and paternal (maternal) constant-region allotypes. Proc. Natl. Acad. Sci. U.S.A. 71: 1169–1173, 1974

    Google Scholar 

  • Knight, K. L. and Hanly, W. C.: Genetic control of a chains of rabbit IgA: Allotypic specificities on the variable and the constant regions. In F. P. Inman and W. J. Mandy (eds.): Contemporary Topics in Molecular Immunology, pp. 55–88, Plenum Press, New York, 1975

    Google Scholar 

  • Lammert, J. M., Dray, S., Knight, K. L., and Hanly, W. C.: Additional rabbit IgA allotypes, f69, f70, g76, g77: Control by the Cαf and Cαg loci. J. Immunol. 118: 1397–1402, 1977

    Google Scholar 

  • Landucci-Tosi, S. and Tosi, R. M.: Recombinant IgG molecules in rabbits doubly heterozygous for group a and group e allotypic specificities. Immunochemistry 10: 65–71, 1973

    Google Scholar 

  • McCartney-Francis, N. and Mandy, W. J.: Serological and chemical studies of latent allotypes in the rabbit. Ann. Immunol. (Paris) 130C: 115–131, 1979

    Google Scholar 

  • Mage, R. G., Young-Cooper, G. O., and Alexander, C.: Genetic control of variable and constant regions of immunoglobulin heavy chains. Nature 230: 63–64, 1971

    Google Scholar 

  • Mage, R. G.: Structure and expression ofVH andCH allotypic determinants. In T. E. Mandel (ed.): Proceedings of the Third International Congress of Immunology: Progress in Immunology III, pp. 289–297, Australian Academy of Sciences, Canberra City, 1977

    Google Scholar 

  • Mandy, W. J. and Todd, C. W.: A11 in rabbits: A specificity detected by agglutination. Immunochemistry 6: 811–823, 1969

    Google Scholar 

  • Mandy, W. J. and Strosberg, A. D.: Latent expression of a Cγ gene. J. Immunol. 120: 1160–1163, 1978

    Google Scholar 

  • Martens, C. L., Gilman-Sachs, A., and Knight, K.: Control of expression of immunoglobulin genes in rabbits. In: C. M. Steinberg and I. Lefkovits (eds.) The Immune System, Vol. 1; S. Karger, Basel in press, 1981

    Google Scholar 

  • Mudgett, M., Fraser, B. A., and Kindt, T. J.: Nonallelic behavior of rabbit variable-region allotypes. J. Exp. Med. 141: 1448–1452, 1975

    Google Scholar 

  • Mudgett-Hunter, M., Yarmush, M. L., Fraser, B. A., and Kindt, T. J.: Rabbit latent group a allotypes: Characterization and relationship to nominal group a allotypic specificities. J. Immunol. 121: 1132–1138, 1978

    Google Scholar 

  • Naessens, J., Hamers-Casterman, C., Hamers, R., and Okerman, F.: IgM allotypes in rabbit. I. a locus associated allotypic determinants. Immunogenetics 6: 17–27, 1978

    Google Scholar 

  • Naessens, J., Hamers-Casterman, C., and Hamers, R.: In vivo suppression ofVH, Cγ, and Cμ gene products in rabbit by maternal immunization against IgM allotypes and detection of somatic recombinant molecules in these animals. Eur. J. Immunol. 10: 776–781, 1980

    Google Scholar 

  • Pernis, B., Forni, L., Dubiski, S., Kelus, A. S., Mandy, W. J., and Todd, C. W.: Heavy chain variable and constant region allotypes in single rabbit plasma cells. Immunochemistry 10: 281–285, 1973

    Google Scholar 

  • Roux, K., Gilman-Sachs, A., Horng, W., and Dray, S.: Additional rabbit IgM allotypes. Effect of allotype suppression of a locus allotypes on n allotypes. Fed. Proc. 38: 1004, 1979

    Google Scholar 

  • Roux, K. H.: A fourth heavy chain variable region subgroup, w, with 2 variants defined by an induced auto-antiserum in the rabbit. J. Immunol. 127: 626–632, 1981

    Google Scholar 

  • Strosberg, A. D., Hamers-Casterman, C., Van der Loo, W., and Hamers, R.: A rabbit with the allotypic phenotype: ala2a3b4b5b6. J. Immunol. 113: 1313–1318, 1974

    Google Scholar 

  • Van der Loo, W., De Baetselier, P., Hamers-Casterman, C., and Harriers, R.: Evidence for quasi-silent germline genes coding for phylogenetically ancient determinants of the rabbit a locus allotypes. Eur. J. Immunol. 7: 15–22, 1977

    Google Scholar 

  • Yarmush, M. L., Mandy, W. J., and Kindt, T. J.: Evidence for linked expression of latent allotypes of the heavy chain constant and variable regions. J. Immunol. 124: 2864–2869, 1980

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20205, Bethesda, Maryland

    Rose G. Mage

  2. Department of Microbiology and Immunology, University of Illinois at the Medical Center, 60680, Chicago, Illinois

    Sheldon Dray, Alice Gilman-Sachs & Katherine L. Knight

  3. Laboratorium Algemene Biologic-Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, St. Genesius Rode, Belgium

    Cécile Hamers-Casterman, Raymond Hamers & Jan Naessen

  4. Department of Preventive Medicine and Community Health, University of Illinois at the Medical Center, 60680, Chicago, Illinois

    W. Carey Hanly

  5. Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20205, Bethesda, Maryland

    Thomas J. Kindt

  6. Department of Microbiology, University of Texas, 78712, Austin, Texas

    William J. Mandy

Authors
  1. Rose G. Mage
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  2. Sheldon Dray
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  3. Alice Gilman-Sachs
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  4. Cécile Hamers-Casterman
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  5. Raymond Hamers
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  6. W. Carey Hanly
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  7. Thomas J. Kindt
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  8. Katherine L. Knight
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  9. William J. Mandy
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  10. Jan Naessen
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Mage, R.G., Dray, S., Gilman-Sachs, A. et al. Rabbit heavy chain haplotypes — Allotypic determinants expressed byVH-CH recombinants. Immunogenetics 15, 287–297 (1982). https://doi.org/10.1007/BF00364337

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  • DOI: https://doi.org/10.1007/BF00364337

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