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Biochemical studies of H-2K antigens from a group of related mutants. II. Identification of a shared mutation in B6-H-2 bm6, B6.C-H-2 bm7, and B6.C-H-2 bm9

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Abstract

In an earlier paper, we presented evidence that two independent mutants of the bg series, B6-H-2 bm5 (bm5) and B6-H-2 bm16 (bm16) carry identical mutations such that tyrosine at residue number 116 of the H-2Kb molecule from the parent strain C57BL/6Kh is replaced by a phenylalanine in each of the two mutant molecules. In this paper, we demonstrate, using similar techniques, that the independent bg series mutants B6-H-2 bm6 (bm6), B6.C-H-2 bm7 (bm7), and B6.C-H-2 bm9 (bm9), which share biological properties with bm5 and bm16, can be grouped together because they share two identical mutations, one of which is common to bm5 and bm16, a Tyr to Phe interchange at residue number 116. In addition, a second mutation is at residue number 121, where a Cys in the H-2K molecule from 136 is substituted with an Arg in the mutant. Since all of the bg series mutants arose independently and share biological and biochemical characteristics, it is anticipated that study of these mutants could lead to some understanding of the high mutation rate in the Kb molecule.

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Abbreviations

B6:

C57BL/6Kh

bm5 :

B6-H-2 bm5

bm6 :

B6-H-2 bm6

bm7 :

B6.C-H-2 bm7

bm9 :

B6.C-H-2 bm9

bm16 :

B6-H-2 bm16

D:

H-2D

K:

H-2K

MHC:

major histocompatibility complex

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Author notes

  1. Karen M. Yamaga

    Present address: Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Honolulu, Hawaii

  2. Tosiki Nisizawa

    Present address: National Institute of Health, Tokyo, Japan

  3. Roger W. Melvold

    Present address: Section of Medical Oncology, Department of Medicine and Microbiology-Immunology, Northwestern University Medical School, 60601, Chicago, Illinois

Authors and Affiliations

  1. Department of Microbiology and Immunology and Department of Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York

    Karen M. Yamaga, Gertrude M. Pfaffenbach, Larry R. Pease, Diane McGovern, Tosiki Nisizawa &  Stanley G. Nathenson

  2. Shields Warren Radiation Laboratory, Harvard Medical School, 02115, Boston, Massachusetts

    Roger W. Melvold & Henry I. Kohn

Authors
  1. Karen M. Yamaga
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  2. Gertrude M. Pfaffenbach
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  3. Larry R. Pease
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  4. Diane McGovern
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  6. Roger W. Melvold
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  7. Henry I. Kohn
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  8. Stanley G. Nathenson
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Yamaga, K.M., Pfaffenbach, G.M., Pease, L.R. et al. Biochemical studies of H-2K antigens from a group of related mutants. II. Identification of a shared mutation in B6-H-2 bm6, B6.C-H-2 bm7, and B6.C-H-2 bm9 . Immunogenetics 17, 31–41 (1983). https://doi.org/10.1007/BF00364287

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  • DOI: https://doi.org/10.1007/BF00364287

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