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Biochemical studies of H-2K antigens from a group of related mutants. I. Identification of a shared mutation in B6-H-2 bm5 and B6-H-2 bm16

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Abstract

Structural studies of the H-2 gene products from a group of five closely related but independent C57BL/6 H-2 mutant mice were undertaken. Each of the mutants exhibits reciprocal graft rejection with the parent. The group is remarkable, however, because each member of this group can accept skin grafts from any other member. The results of biochemical analysis of the H-2 glycoproteins from two of these related mutants, bm5 and bm16, are presented in this report. Evidence is given that the H-2K molecules from these two mutants are identical to each other based on comparative tryptic peptide mapping profiles with the parent. From partial amino acid sequence analysis, K products of both mutants have at least one common difference from the parental type located at residue number 116. Definitive studies established that in both bm5 and bm16 a tryosine found in the parent molecule is substituted with a phenylalanine in the mutant. These results show that a biochemical difference between the K products of the two mutants and of the parent can be detected, that the mutants appear to be identical with one another even though they arose independently, and that they differ from the other H-2K b mutants analyzed.

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Abbreviations

B6:

C57BL/6Kh

bm5 :

B6-H-2bm5

bm6 :

B6-H-2 bm6

bm7 :

B6.C-H-2 bm7

bm9 :

B6.C-H-2 bm9

bm16 :

B6-H-2 bm16

D:

H-2D

K:

H-2K

MHC:

major histocompatibility complex

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Author notes

  1. Karen M. Yamaga

    Present address: Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Honolulu, Hawaii

  2. Tosiki Nisizawa

    Present address: National Institute of Health, Tokyo, Japan

  3. Roger W. Melvold

    Present address: Section of Medical Oncology, Department of Medicine and Microbiology-Immunology, Northwestern University Medical School, 60601, Chicago, Illinois

Authors and Affiliations

  1. Department of Microbiology and Immunology and Department of Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York

    Karen M. Yamaga, Gertrude M. Pfaffenbach, Larry R. Pease, Diane Mc Governs, Tosiki Nisizawa &  Stanley G. Nathenson

  2. Shields Warren Radiation Laboratory, Harvard Medical School, Boston, Massachusetts

    Roger W. Melvold & Henry I. Kohn

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  1. Karen M. Yamaga
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  2. Gertrude M. Pfaffenbach
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  3. Larry R. Pease
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  4. Diane Mc Governs
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  6. Roger W. Melvold
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  7. Henry I. Kohn
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  8. Stanley G. Nathenson
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Yamaga, K.M., Pfaffenbach, G.M., Pease, L.R. et al. Biochemical studies of H-2K antigens from a group of related mutants. I. Identification of a shared mutation in B6-H-2 bm5 and B6-H-2 bm16 . Immunogenetics 17, 19–29 (1983). https://doi.org/10.1007/BF00364286

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  • DOI: https://doi.org/10.1007/BF00364286

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