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Mikropunktionsuntersuchungen am proximalen und distalen Konvolut der Rattenniere über den Einfluß von Actinomycin D aufden mineralocorticoidabhängigen Na-Transport

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Summary

In micropuncture experiments the effect of actinomycin D on the transtubular Na reabsorption in the proximal and distal convolution of the rat kidney was investigated using the split oil droplet method.

1. In control rats with intact adrenals actinomycin D (dose: 200 μg/100 g b.w. intravenously) inhibited the rate of Na reabsorption in both segments. The inhibition could be demonstrated 60 min after administration of the antibiotic, maximum response was seen 90–120 min after actinomycin injection.

2. A similar inhibition could be observed in adrenalectomized rats pretreated with d-aldosterone (dose 7.5 μg/100 g b.w. and 24 hrs) for 3–5 days.

3. In adrenalectomized rats without hormone substitution actinomycin D had no effect on Na reabsorption in either segment.—The results demonstrate that in the mammalian kidney only that fraction of Na transport can be blocked by actinomycin D, which is influenced by mineralocorticosteroids. The steroid independent fraction is unaffected by the antibiotic. The actinomycin D effects constitute further proof that in the proximal as in the distal convolution a fraction of Na transport is under the influence of mineralocorticosteroids. The results are compatible with the hypothesis that d-aldosterone influences Na transport in the rat kidney as in isolated membranes by affecting nuclear metabolism.

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  1. Physiologisches Institut der Freien Universität Berlin, Germany

    M. Wiederholt

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  1. M. Wiederholt
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Wiederholt, M. Mikropunktionsuntersuchungen am proximalen und distalen Konvolut der Rattenniere über den Einfluß von Actinomycin D aufden mineralocorticoidabhängigen Na-Transport. Pflügers Archiv 292, 334–342 (1966). https://doi.org/10.1007/BF00363201

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  • DOI: https://doi.org/10.1007/BF00363201

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