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An improvement in Pichia stipitis fermentation of acid-hydrolysed hemicellulose achieved by overliming (calcium hydroxide treatment) and strain adaptation

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Abstract

The fermentability of a corn cob, acid-hydrolysed hemicellulose by Pichia stipitis was considerably improved by pre-treatment with Ca(OH)2. The total sugars utilized and ethanol yield for the untreated hydrolysate were 18% and 0.21 g/g, respectively, compared with 82% and 0.32 g/g respectively for the treated material. Adaptation of the yeast to the hydrolysate resulted in a significantly higher fermentation rate with over 90% of the initial total sugars being utilized and an ethanol yield and maximum ethanol concentration of 0.41 g/g and 13.3 g/l, respectively.

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The authors are with the USDA Forest Products Laboratory. One Gifford Pinchot Drive. Madison, WI, 53705 USA

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Amartey, S., Jeffries, T. An improvement in Pichia stipitis fermentation of acid-hydrolysed hemicellulose achieved by overliming (calcium hydroxide treatment) and strain adaptation. World Journal of Microbiology & Biotechnology 12, 281–283 (1996). https://doi.org/10.1007/BF00360928

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  • DOI: https://doi.org/10.1007/BF00360928

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