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Production of amyloglucosidase by Aspergillus niger under different cultivation regimens

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Abstract

Amyloglucosidase (AMG) was produced by Aspergillus niger in solid-state fermentation (SSF), submerged fermentation (SmF) and an aqueous, two-phase system of polyethyleneglycol (PEG) and salt. In SSF, a fed-batch mode of operation gave a yield of 64 U/ml compared with 44 U/ml in batch mode. Similar trends were observed for SmF, where fed-batch cultivation gave a yield of 102 U/ml compared with 66 U/ml in batch. Shorter cultivation times (66 h) were required for SmF than for SSF (96 h). In the aqueous, two-phase cultivation, the productivity and yield of AMG were both twice those in the control fermentation.

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References

  • AlazardD. & RaimbauldM. 1981 Comparative study of amylolytic enzymes production by Aspergillus niger in liquid and solidstate cultivation. European Journal of Applied Microbiology and Biotechnology 12, 113–117.

    Google Scholar 

  • AliS., MahmoodS., AlamR. & HossainZ. 1989 Culture condition for production of glucoamylase from rice bran by Aspergillus terreus. MIRCEN Journal of Applied Microbiology and Biotechnology 5, 525–532.

    Google Scholar 

  • AnderssonE., JohanssonA.C. & Hahn-HägerdalB. 1985 Alpha amylase production in aqueous two-phase systems with Bacillus subtilis. Enzyme and Microbial Technology 7, 333–338.

    Google Scholar 

  • BandyopadhyayA.K. 1988 Production of glucoamylase by Aspergillus oryzae in different media and under various culturing conditions. Indian Drugs 26, 46–50.

    Google Scholar 

  • BarnesW.C. & RydalmereA.B.B. 1974 Determination of cereal alpha amylase using a commercially available dye labelled substrate. Starke/Stärch 26, 193–197.

    Google Scholar 

  • BayerK. & DierO.D. 1984 Optimizing fungal amylase production in submerged culture. In Proceedings of Third European Congress on Biotechnology, Vol. 1, pp. 725–730. Weinheim: Verlag Chemie.

    Google Scholar 

  • BernfeldP. 1955 Methods in Enzymology 1, 149–158.

    Google Scholar 

  • InshikP. & ChungY. 1989 Some factors affecting glucoamylase production from Aspergillus sp. Korean Journal of Applied Microbiology and Bioengineering 17, 519–523.

    Google Scholar 

  • KaulR. & MattiassonB. 1991 Extractive bioconversion in aqueous two-phase system. In Extractive Bioconversions, eds MattiassonB. & HolstO. pp. 173–188. New York: Marcel Dekker.

    Google Scholar 

  • KumarP.K.R. & LonsaneB.K. 1987 Potential of fed-batch culture in solid-state fermentation for production of gibberelic acid. Biotechnology Letters 9, 179–182.

    Google Scholar 

  • LonsaneB.K. & RameshM.V. 1990 Production of bacterial thermostable alpha amylase by solid-state fermentation, a potential tool for achieving economy in enzyme production and starch hydrolysis. Advances in Applied Microbiology 35, 1–55.

    Google Scholar 

  • PandeyA. 1991a Recent process developments in solid-state fermentation. Process Biochemistry 27, 109–117.

    Google Scholar 

  • PandeyA. 1991b Effect of particle size of substrate on enzyme production in solid-state fermentation. Bioresource Technology 37, 169–172.

    Google Scholar 

  • PandeyA. & RadhakrishnanS. 1993 The production of glucoamylase by Aspergillus niger NCIN 1245. Process Biochemistry 28, 305–309.

    Google Scholar 

  • PerssonI., TjerneldF. & Hahn-HägerdalB. 1984 Semicontinuous cellulase production in an aqueous two-phase system with Trichoderma reesei Rutgers C30. Enzyme and Microbial Technology 6, 415–418.

    Google Scholar 

  • RamamurthyY. & KothariR.M. 1993 Comparison of fungal protease production by submerged and surface cultivation. Journal of Biotechnology 27, 349–354.

    Google Scholar 

  • Solis-PereiraS., Favela-TorresE., Viniegra-GonzalezG. & Gutierrez-RojasM. 1993 Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid-state fermentations. Applied Microbiology and Biotechnology 39, 36–41.

    Google Scholar 

  • YongK.P. & DeSantiM.S.S. 1977 Introduction of high amyloglucosidase producing mutant from Aspergillus awamori. Journal of Fermentation Technology 55, 192–195.

    Google Scholar 

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Authors
  1. M. Ramadas
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  2. O. Holst
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  3. B. Mattiasson
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Additional information

M. Ramadas is with the Department of Biochemistry, Faculty of Medicine, University of Jaffna, Kokuvil, Sri Lanka. O. Holst and B. Mattiasson are with the Department of Biotechnology, Chemical Center, Lund University, Box 124, S-221 00 Lund, Sweden

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Ramadas, M., Holst, O. & Mattiasson, B. Production of amyloglucosidase by Aspergillus niger under different cultivation regimens. World Journal of Microbiology & Biotechnology 12, 267–271 (1996). https://doi.org/10.1007/BF00360926

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  • DOI: https://doi.org/10.1007/BF00360926

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