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Elements of the secondary structure in the DNA, complementary to pigeon globin mRNA

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Abstract

Pigeon globin mRNA isolated from reticulocyte polysomes was copied by means of RNA-directed DNA-polymerase in the presence of actinomycin d. Hydroxyapatite chromatography with and without formaldehyde and hydrolysis with S1 nuclease were used for evaluation of the secondary structure elements in cDNA synthesized by reverse transcription.

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Abbreviations

cDNA:

DNA complementary to mRNA synthesized by reverse transcription

HAP:

hydroxyapatite

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Authors and Affiliations

  1. I. V. Kurchatov Institute of Atomic Energy, Institute of Molucular Biology, Academy of Sciences of the U.S.S.R., Moscow, U.S.S.R.

    K. G. Gasaryan, V. Z. Tarantul, Yu. N. Baranov, L. Yu. Frolova & L. L. Kisselev

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  1. K. G. Gasaryan
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  2. V. Z. Tarantul
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  3. Yu. N. Baranov
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  4. L. Yu. Frolova
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  5. L. L. Kisselev
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Gasaryan, K.G., Tarantul, V.Z., Baranov, Y.N. et al. Elements of the secondary structure in the DNA, complementary to pigeon globin mRNA. Molecular Biology Reports 1, 465–470 (1974). https://doi.org/10.1007/BF00360673

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  • DOI: https://doi.org/10.1007/BF00360673

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