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Poly a blocks in the nuclear ribonucleoprotein complexes, containing pre-mRNA

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Abstract

Poly A was found in nuclear particles containing pre-mRNA. It was shown that during the isolation of 30S particles from rat liver or Ehrlich ascites carcinoma nuclei, all poly A is detached from the particles containing pre-mRNA and is found in the form of RNP with a sedimentation coefficient of about 14S. When RNP polyparticles are isolated in the presence of RNase inhibitor poly A is distributed among the particles of higher molecular weights.

Since the sedimentation properties and buoyant density of the poly A-containing particles are different from the 30S particles it was suggested that the poly A fragments are bound not with informofers, but with another kind of protein.

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Author notes

  1. J. Besson

    Present address: Dept. of Physical Biology, Institute of Medicine, Lyon, France

Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the U.S.S.R., Moscow, USSR

    O. P. Samarina, N. A. Aitkhozhina & J. Besson

Authors
  1. O. P. Samarina
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  2. N. A. Aitkhozhina
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  3. J. Besson
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Samarina, O.P., Aitkhozhina, N.A. & Besson, J. Poly a blocks in the nuclear ribonucleoprotein complexes, containing pre-mRNA. Molecular Biology Reports 1, 193–199 (1973). https://doi.org/10.1007/BF00357641

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  • DOI: https://doi.org/10.1007/BF00357641

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