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Studies on chromatin

III. v-Bodies and Free DNA in Chromatin Lacking Histone H1

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Abstract

Chromatin lacking histone H1 was found by electron microscopy to contain ‘beaded’ deoxyribonucleoprotein fibers. Adjacent beads are connected with each other by threads having a DNA-like appearance. At least some of threads are shown to be free DNA stretches. Average length and the content of free DNA stretches in histone H1-depleted chromatin depends on the ionic conditions of the medium. The appearance of individual beads is similar to that of chromatin subunits or v-bodies [1] in the original chromatin.

Thus, in agreement with the X-ray data [2], histone H1 apparently is not required for maintenance of a compact state of DNA in chromatin subunits.

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Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the U.S.S.R., Moscow, USSR

    A. J. Varshavsky & V. V. Bakayev

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  1. A. J. Varshavsky
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  2. V. V. Bakayev
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Varshavsky, A.J., Bakayev, V.V. Studies on chromatin. Molecular Biology Reports 2, 209–217 (1975). https://doi.org/10.1007/BF00356990

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  • DOI: https://doi.org/10.1007/BF00356990

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