Abstract
The mouse major histocompatibility complex (MHC) contains many genes in addition to the classical immune response genes. We have screened overlapping cosmid clones covering 170 kb of the H-2K region for genes expressed in embryonal carcinoma (EC) cells. The Ke-3 gene (Abe et al. 1988) found in this region was further studied by Southern, Northern, and sequence analysis. It is an expressed, intron-containing locus encoding a mouse homolog of the bacterial ribosomal protein S13. This is the first nonorganelle S13 homolog identified in metazoans, and its genomic location has been determined precisely.
Similar content being viewed by others
References
Abe, K., Wei, J.-F., Wei, F.-S., Hsu, Y.-C., Uehara, H., Artzt, K., and Bennett, D.: Searching for coding sequences in the mammalian genome: The H-2K region of the mouse MHC is replete with genes expressed in embryos. EMBO J 7:3441–3449, 1988.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J.: Basic local alignment search tool. J Mol Biol 215:403–410, 1990.
Artzt, K., Abe, K., Uehara, H., and Bennett, D.: Intra-H-2 recombination in the t haplotypes shows a hot spot and close linkage of ltw5 to H-2K. Immunogenetics 28:30–37, 1988.
Committee for Mouse Chromosome 17 {Artzt, K., Barlow, D., Dove, W., Fischer-Lindahl, K., Klein, J., Lyon, M.F. and Silver, L.M.}: Maps of mouse chromosome 17: First report. Mammalian Genome 1: 5–29, 1991.
D'Eustachio, P., Meyuhas, O., Ruddle, F., and Perry, R.P.: Chromosomal distribution of ribosomal protein genes in the mouse. Cell 24: 307–312, 1981.
Dudov, K.P. and Perry, R.P.: The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene. Cell 37: 457–468, 1984.
Feinberg, A.P. and Vogelstein, B.: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983.
Fisher, E.M.C., Beer-Romero, P., Brown, L.G., Ridley, A., McNeil, J.A., Lawrence, J.B., Willard, H.F., Bieber, F.R., and Page, D.C.: Homologous ribosomal protein genes on the human X and Y chromosomes: Escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205–1218, 1990.
Giri, L., Hill, W.E., and Wittmann, H.G.: Ribosomal proteins: Their structure and spatial arrangement in prokaryotic ribosomes. Adv Protein Chem 36: 1–78, 1984.
Goldbard, S.B., Verbanac, K.M., and Warner, C.M.: Genetic analysis of H-2 linked gene(s) affecting early mouse embryo development. J Immunogenet 9: 77–82, 1982.
Henkin, T.M. and Chambliss, G.H.: Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4. Mol Gen Genet 193: 364–369, 1984.
Kongsuwan, K., Yu, Q., Vincent, A., Frisardi, M.C., Rosbash, M., Lengyel, J.A., and Merriam, M.: A Drosophila Minute gene encodes a ribosomal protein. Nature 317: 555–558, 1985.
Kozak, M.: Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44: 283–292, 1986.
MacMurray, A. and Shin, H.-S.: The antimorphic nature of the T c allele at the mouse T locus. Genetics 120: 545–550, 1988.
Monk, R.J., Meyuhas, O., and Perry, R.P.: Mammals have multiple genes for individual ribosomal proteins. Cell 24: 301–306, 1981.
Nelson, K.K. and Green, M.R.: Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing. Genes Develop 2: 319–329, 1988.
Reed, K.C. and Mann, D.A.: Rapid transfer of DNA from agarose gels to nylon membranes. Nucl Acids Res 13: 7207–7221, 1985.
Shin, H.-S., Flaherty, L., Artzt, K., Bennett, D., and Ravetch, J.: Inversion in the H-2 complex of t-haplotypes in mice. Nature 306: 380–383, 1983.
St.-Jacques, B., Han, T.-H., MacMurray, A., and Shin, H.-S.: A putative transmembrane protein with histidine-rich charge clusters encoded in the H-2K/tw5 region of mice. Mol Cell Biol 10: 138–145, 1990.
Uehara, H., Abe, K., Park, C.-H., Shin, H.-S., Bennett, D., and Artzt, K.: The molecular organization of the H-2K region of two t-haplotypes: Implications for the evolution of genetic diversity. EMBO J 6: 83–90, 1987.
Wagner, M. and Perry, R.P.: Characterization of the multigene family encoding the mouse S16 ribosomal protein: Strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA. Mol Cell Biol 5: 3560–3576, 1985.
Wiedemann, L.M. and Perry, R.P.: Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family. Mol Cell Biol 4: 2518–2528, 1984.
Wiedemann, L.M., D'Eustachio, P., Kelley, D.E., and Perry, R.P.: Three functional ribosomal protein genes are unlinked in mouse genome. Som Cell Mol Genet 13: 77–80, 1987.
Wool, I.G., Endo, Y., Chan, Y.-L., and Glück, A.: Studies of the structure, function, and evolution of mammalian ribosomes. In W.E. Hill (ed.); Structure, Function, and Evolution of Ribosomes, pp. 203–214, American Society of Microbiology, Washington, D.C., 1990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MacMurray, A.J., Shin, HS. The murine MHC encodes a mammalian homolog of bacterial ribosomal protein S13. Mammalian Genome 2, 87–95 (1992). https://doi.org/10.1007/BF00353855
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00353855