Skip to main content
Log in

The murine MHC encodes a mammalian homolog of bacterial ribosomal protein S13

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

The mouse major histocompatibility complex (MHC) contains many genes in addition to the classical immune response genes. We have screened overlapping cosmid clones covering 170 kb of the H-2K region for genes expressed in embryonal carcinoma (EC) cells. The Ke-3 gene (Abe et al. 1988) found in this region was further studied by Southern, Northern, and sequence analysis. It is an expressed, intron-containing locus encoding a mouse homolog of the bacterial ribosomal protein S13. This is the first nonorganelle S13 homolog identified in metazoans, and its genomic location has been determined precisely.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Abe, K., Wei, J.-F., Wei, F.-S., Hsu, Y.-C., Uehara, H., Artzt, K., and Bennett, D.: Searching for coding sequences in the mammalian genome: The H-2K region of the mouse MHC is replete with genes expressed in embryos. EMBO J 7:3441–3449, 1988.

    Google Scholar 

  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J.: Basic local alignment search tool. J Mol Biol 215:403–410, 1990.

    Google Scholar 

  • Artzt, K., Abe, K., Uehara, H., and Bennett, D.: Intra-H-2 recombination in the t haplotypes shows a hot spot and close linkage of ltw5 to H-2K. Immunogenetics 28:30–37, 1988.

    Google Scholar 

  • Committee for Mouse Chromosome 17 {Artzt, K., Barlow, D., Dove, W., Fischer-Lindahl, K., Klein, J., Lyon, M.F. and Silver, L.M.}: Maps of mouse chromosome 17: First report. Mammalian Genome 1: 5–29, 1991.

    Google Scholar 

  • D'Eustachio, P., Meyuhas, O., Ruddle, F., and Perry, R.P.: Chromosomal distribution of ribosomal protein genes in the mouse. Cell 24: 307–312, 1981.

    Google Scholar 

  • Dudov, K.P. and Perry, R.P.: The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene. Cell 37: 457–468, 1984.

    Google Scholar 

  • Feinberg, A.P. and Vogelstein, B.: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983.

    Google Scholar 

  • Fisher, E.M.C., Beer-Romero, P., Brown, L.G., Ridley, A., McNeil, J.A., Lawrence, J.B., Willard, H.F., Bieber, F.R., and Page, D.C.: Homologous ribosomal protein genes on the human X and Y chromosomes: Escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205–1218, 1990.

    Google Scholar 

  • Giri, L., Hill, W.E., and Wittmann, H.G.: Ribosomal proteins: Their structure and spatial arrangement in prokaryotic ribosomes. Adv Protein Chem 36: 1–78, 1984.

    Google Scholar 

  • Goldbard, S.B., Verbanac, K.M., and Warner, C.M.: Genetic analysis of H-2 linked gene(s) affecting early mouse embryo development. J Immunogenet 9: 77–82, 1982.

    Google Scholar 

  • Henkin, T.M. and Chambliss, G.H.: Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4. Mol Gen Genet 193: 364–369, 1984.

    Google Scholar 

  • Kongsuwan, K., Yu, Q., Vincent, A., Frisardi, M.C., Rosbash, M., Lengyel, J.A., and Merriam, M.: A Drosophila Minute gene encodes a ribosomal protein. Nature 317: 555–558, 1985.

    Google Scholar 

  • Kozak, M.: Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44: 283–292, 1986.

    Google Scholar 

  • MacMurray, A. and Shin, H.-S.: The antimorphic nature of the T c allele at the mouse T locus. Genetics 120: 545–550, 1988.

    Google Scholar 

  • Monk, R.J., Meyuhas, O., and Perry, R.P.: Mammals have multiple genes for individual ribosomal proteins. Cell 24: 301–306, 1981.

    Google Scholar 

  • Nelson, K.K. and Green, M.R.: Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing. Genes Develop 2: 319–329, 1988.

    Google Scholar 

  • Reed, K.C. and Mann, D.A.: Rapid transfer of DNA from agarose gels to nylon membranes. Nucl Acids Res 13: 7207–7221, 1985.

    Google Scholar 

  • Shin, H.-S., Flaherty, L., Artzt, K., Bennett, D., and Ravetch, J.: Inversion in the H-2 complex of t-haplotypes in mice. Nature 306: 380–383, 1983.

    Google Scholar 

  • St.-Jacques, B., Han, T.-H., MacMurray, A., and Shin, H.-S.: A putative transmembrane protein with histidine-rich charge clusters encoded in the H-2K/tw5 region of mice. Mol Cell Biol 10: 138–145, 1990.

    Google Scholar 

  • Uehara, H., Abe, K., Park, C.-H., Shin, H.-S., Bennett, D., and Artzt, K.: The molecular organization of the H-2K region of two t-haplotypes: Implications for the evolution of genetic diversity. EMBO J 6: 83–90, 1987.

    Google Scholar 

  • Wagner, M. and Perry, R.P.: Characterization of the multigene family encoding the mouse S16 ribosomal protein: Strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA. Mol Cell Biol 5: 3560–3576, 1985.

    Google Scholar 

  • Wiedemann, L.M. and Perry, R.P.: Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family. Mol Cell Biol 4: 2518–2528, 1984.

    Google Scholar 

  • Wiedemann, L.M., D'Eustachio, P., Kelley, D.E., and Perry, R.P.: Three functional ribosomal protein genes are unlinked in mouse genome. Som Cell Mol Genet 13: 77–80, 1987.

    Google Scholar 

  • Wool, I.G., Endo, Y., Chan, Y.-L., and Glück, A.: Studies of the structure, function, and evolution of mammalian ribosomes. In W.E. Hill (ed.); Structure, Function, and Evolution of Ribosomes, pp. 203–214, American Society of Microbiology, Washington, D.C., 1990.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

MacMurray, A.J., Shin, HS. The murine MHC encodes a mammalian homolog of bacterial ribosomal protein S13. Mammalian Genome 2, 87–95 (1992). https://doi.org/10.1007/BF00353855

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00353855

Keywords

Navigation