Summary
Two PstI fragments (5.3x106 and 4.3x106 daltons) coding for Anacystis nidulans rRNA genes were cloned. The cloned rDNAs were characterized by restriction endonuclease mapping, DNA-RNA hybridization analysis and the R-loop technique. The results indicated that both fragments contained 16S, 23S and 5S rRNA genes in this order. A tRNA gene(s) was detected in the spacer region between 16S and 23S rRNA genes. The organization of A. nidulans rRNA genes resembles those of E. coli and of Euglena chloroplasts rather than those of higher plant chloroplasts.
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Tomioka, N., Shinozaki, K. & Sugiura, M. Molecular cloning and characterization of ribosomal RNA genes from a blue-green alga, Anacystis nidulans . Mol Gen Genet 184, 359–363 (1981). https://doi.org/10.1007/BF00352505
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DOI: https://doi.org/10.1007/BF00352505