Summary
A gene homologous to the E. coli groEL locus was identified on the plastid genome of the unicellular red alga Cyanidium caldarium strain 14-1-1 (synonym: Galdieria sulphuraria). The complete nucleotide sequence was determined and compared to bacterial-and nuclear-encoded counterparts of higher plants. At the amino-acid level the C. caldarium gene shows 70% homology to the corresponding gene of the cyanobacterium Synechococcus and 52% homology to nuclear-encoded counterparts of higher plants, respectively. Northern and Western blot experiments were used to investigate the dependence of the transcript-and protein-level on culture temperature and heat shock.
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Maid, U., Steinmüller, R. & Zetsche, K. Structure and expression of a plastid-encoded groEL homologous heat-shock gene in a thermophilic unicellular red alga. Curr Genet 21, 521–525 (1992). https://doi.org/10.1007/BF00351663
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DOI: https://doi.org/10.1007/BF00351663