Abstract
A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process.
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Abbreviations
- CS:
-
Sodium cellulose sulphate
- CMC:
-
Sodium carboxymethyl-cellulose
- DMEM:
-
Dulbecco's modified Eagle medium
- FCS:
-
fetal calf serum
- mAb:
-
Monoclonal antibodies
- PBS:
-
Phosphate buffered saline
- PDMDAAC:
-
Poly-dimethyl-diallyl-ammoniumchloride
- PEI:
-
Poly-ethylene-imine
- PLL:
-
Poly-L-lysine
- aqua dest.:
-
distilled water
References
ChenTR (1977) In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell. Res. 104: 255–262.
GharapetianH, DaviesNA & SunAM (1986) Encapsulation of viable cells within polyacrylate membranes. Biotechnol. Bioeng. 28: 1595–1600.
GharapetianH, MalekiM, O'SheaGM, CarpenterRC & SunAM (1987) Polyacrylate microcapsules for cell encapsulation: Effects of copolymer structure on membrane properties. Biotechnol. Bioeng. 30: 775–779.
GlackenMW, FleischakerRJ & SinskeyAJ (1983) Mammalian cell culture: Engineering principles and scale-up. Trend Biotechnol. 1: 102–108.
Grdina TA & Jarvis AP Jr (1984) Microencapsulation of human x human hybridoma cells: Cell growth & monoclonal antibody production. Presented at Biotech '84 U.S.A. In: Online Publications, pp. A235–A246, Pinner U.K.
HulstAC, TramperJ, van'tRietK & WesterbeekJMM (1985) A new technique for the production of immobilized biocatalyst in large quantities. Biotechnol. Bioeng. 27: 870–876.
JarvisAPJr & GrdinaTA (1983) Production of biologicals from microencapsulated living cells. Biotechniques 1: 22–27.
KingGA, DaugulisAJ, FaulknerP & GoosenMFA (1987) Alginate-polylysine microcapsules of controlled membrane molecular weight cutoff for mammalian cell culture engineering. Biotechnol. Progr. 3: 231–240.
LimF & SunAM (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210: 908–910.
MertenO-W (1988) Batch production and growth kinetics of hybridomas. Cytotechnology 1: 113–121.
Merten O-W & Dautzenberg H (1987) A new method for the encapsulation of mammalian cells. Presented at the EFB-W.P. Animal and Plant Cell Culture Technology on ‘Standardization of Methodology for Comparison of Reactors as a part of Bioprocesses’. 22–23.10.87, Paris, France.
PhillipB & WagenknechtW (1983) Cellulose sulfate half-ester. Synthesis, structure and properties. Cell. Chem. Technol. 17: 443–459.
PommereningK, RistauO, ReinH, DautzenbergH & LothF (1983) Immobilisierung von Proteinen und Zellfragmenten durch ein neues Verfahren der Mikrokapsulierung. Biomed. Biochim. Acta 42: 813–823.
SeftonMV, DawsonRM, BroughtonRL, BlysniukJ & SugamoriME (1987) Microencapsulation of mammalian cells in a water-insoluble polyacrylate by coextrusion and interfacial precipitation. Biotechnol. Bioeng. 29: 1135–1143.
YoshiokaT, HiranoR, ShioyaT & KakoM (1990) Encapsulation of mammalian cell with chitosan-CMC capsules. Biotechnol. Bioeng. 35: 66–72.
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Merten, O.W., Dautzenberg, H. & Palfi, G.E. A new method for the encapsulation of mammalian cells. Cytotechnology 7, 121–130 (1991). https://doi.org/10.1007/BF00350918
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DOI: https://doi.org/10.1007/BF00350918