Abstract
After enzymic hydrolysis of polysaccharides in milled wood or pulp the unhydrolysed lignin residue becomes soluble in certain polar solvents. Extensive disintegration of the wood is necessary to obtain a sufficient accessibility toward the enzyme. For milling a porcelain rotary ball mill is recommended. A relationship has been found between the milling energy, which is proportional to the size of the mill, the milling time, and the yield of ball-milled wood lignin (BMWL). Enzymatically isolated lignin (EIL) requires a critical milling time which is shorter than that for obtaining maximum yields of BMWL. A procedure for isolation of the total lignin content from wood and pulps in the form of three fractions has been developed, giving a water soluble, low molecular carbohydrate-lignin complex by water extraction of ball-milled wood, a BMWL-carbohydrate complex by subsequent extraction with dioxane, and a EIL-carbohydrate complex by dioxane extraction of the residue after enzymic hydrolysis.
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Azhar, R. M.; Polčin, J.; Rapson, W. H. 1971. Characterization of enzymatically isolated lignin. Paper presented at the Annual Meeting of TAPPI, New York, Febr. 21–25
Bezúch, B.; Polčin, J. (1978. structural studies of EIL from hardwoods. Papir a celul. 33: 29–32
Björkman, A. 1956. Studies on finely divided wood, 1. Svensk Papperstid. 59: 477–485
Brauns, F. E. 1952. The chemistry of lignin. Academic Press, New York
Brauns, F. E.; Brauns, D. A. 1960. The chemistry of lignin. Suppl. Vol. Academic Press, New York, 62–66
Brownell, H. H. 1965. Isolation of milled wood lignin and lignin-carbohydrate complex. Tappi 48: 513–519
Chang, H-M.; Cowling, E. B.; Brown, W.; Adler, E.; Miksche, G. 1975. Comparative studies on cellulolytic enzyme lignin and MWL of sweetgum and spruce. Holzforschung 29: 153–159
Košikova, B.; Polčin, J.; Dandarova, M.; Budiska, E. 1968. The study on molecular grinding of spruce wood with regard to liberation of lignin. Drev. výsk. 1: 25–35
Lai, Y. Z.; Sarkanen, K. V. 1971. Isolation and structural studies. In: Sarkanen, K. V., Ludwig, C. H. (Eds.). Lignins. Wiley-Interscience, New York, 165–240
Pew, J. C. 1957. Properties of powdered wood and isolation of lignin by cellulytic enzymes. Tappi 40: 553–558
Pew, J. C.; Weyna, P. 1962. Fine grinding, enzyme digestion, and the lignin-cellulose bond in wood. Tappi 45: 247–256
Polčin, J.; Bezúch, B. 1973. Enzymatic isolation of lignin, Part I. 14–58. Rep. PPRI, Bratislava
Polčin, J.; Bezúch, B. 1977. Investigation on enzymic hydrolysis of lignified cellulosic materials. Wood Sci. Technol. 11: 275–290
Shubert, W. J. 1965. Lignin Biochemistry. Academic Press, New York. 8–12, 78–117
Somogyi, M. 1952. Notes on sugar determination. J. Biol. Chem. 195: 19–23
Wayman, M.; Obiaga, T. I. 1974. Molecular weights of milled-wood lignins. Tappi 57: 123–126
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Polčin, J., Bezúch, B. Enzymic isolation of lignin from wood and pulps. Wood Sci. Technol. 12, 149–158 (1978). https://doi.org/10.1007/BF00350820
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DOI: https://doi.org/10.1007/BF00350820