Summary
The pictures of isolated mitochondrial membranes, as seen on the electron-microscope, depend very much on the method of specimen preparation. Subunits of linear dimensions of about 25 mμ, (electron transport particles) are observed in carbon-replicas of the membranes and in specimens treated with trypsin or pepsin (0.02% for 30 mins) and shadowed with platinum. A three-layered structure of the unit membrane is seen in sections of specimens fixed with osmium tetroxide or formalin followed by post-fixation with osmium tetroxide. But fixation with potassium permanganate or with formalin, followed by post-fixation with potassium permanganate reveals an electron-dense “globular structural element” in the unit membrane. An electron-transparent “ultrastructural element” of the unit membrane is observed after treatment with trypsin (0.2% for 5 mins) and fixation with osmium tetroxide. Unsectioned specimens treated with 0.02% trypsin for 30 mins show a honeycomb-like structure of the membrane. Thus, part of the results appear to support the concept of a mosaic-like structure of the unit membrane, whereas other results are in agreement with the “classical” concept of a three-layered structure.
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Deutsch, K., W. Krause, and S. Rosenthal: An electron microscopical study of isolated mitochondrial membranes treated with osmium tetroxide, potassium permanganate and formaldehyde. Quart. J. micr. Sci. 105, 319–323 (1964).
Fernández-Morán, H.: Cell-membrane ultrastructure. Circulation 26, 1039–1065 (1962).
—: Membranes, biological. McGraw-Hill yearbook of science and technology, p. 323–329. New York: McGraw-Hill Book. Co. 1963.
Green, D. E.: The mitochondrion. Sci. American 210, 63 (1964).
—, P. V. Blair, and T. Oda: Isolation and characterization of the unit of electron transfer in heart mitochondria. Science 140, 382 (1963).
—, u. T. Oda: Die Struktur und Funktion der subcellulären Partikel. Pharmazie 17, 567–588 (1962).
Haggis, G. H., D. Michie, A. R. Muir, K. B. Roberts, and P. M. B. Walker: Introduction to molecular biology, p. 187. London: Longmans 1964.
Kellenberger, E., R. Schwab, et A. Ryter: L'utilisation d'un copolymère de groupe des polyester comme matériel d'inclusion en ultramicrotomie. Experientia (Basel) 12, 421–442 (1956).
Krause, W., u. K. Deutsch: Ein vernetztes Polymethylacrylat als Einbettungsmittel für elektronenmikroskopische Untersuchungen. Medizintechnik 5, 124–125 (1965).
Kushida, H.: A new embedding method for ultra thin sectioning using a methacrylate resin with three dimensional polymer structure. J. Electronmicroscopy (Chiba) 10, 194–197 (1961).
Lehninger, A. L.: Respiratory energy transformation. Review of Mod. Physics 31, 136–149 (1959).
Linnane, A. W., and D. M. Ziegler: Studies on the mechanism of oxydative phosphorylation. Biochim. biophys. Acta (Amst.) 29, 630–638 (1958).
Palade, G. E.: The fine structure of mitochondria. Anat. Rec. 114, 427–451 (1952).
Parsons, D. F.: Mitochondrial structure: two types of subunitson negatively stained mitochondrial membranes. Science 140, 985–987 (1963).
Pease, D. C.: Buffered formaldehyde as a killing agent and a primary fixative for electron microscopy. Anat. Rec. 142, 342 (1962).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Robertson, J. D.: The ultrastructure of cell membranes. Biochemical Society Symposia No 16, p. 3–43. Cambridge: University Press (1959).
Sabatini, D. D., C. Bensch, and R. J. Barnett: The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Sjösteand, F. S.: Ultrastructure of rod-shaped mitochondria. Nature (Lond.) 171, 30 (1953).
—: A new unltrastructural element of the membranes in mitochondria and of some cytoplasmic membranes. J. Ultrastruct. Res. 9, 340–361 (1963).
— E. Anderson Cedergren, and V. Karlson: Myelin-like figures formed from mitochondrial material. Nature (Lond.) 1075–1078 (1964).
Stoeckenius, W.: Some observations on negatively stained mitochondria. J. Cell Biol. 17, 443–454 (1963).
Ziegler, D. M., A. W. Linnane, D. E. Green, C. M. E. Dass, and H. Ris: Correlation of the morphology and enzymatic properties of the mitochondrial and submitochondrial particles. Biochim. biophys. Acta. (Amst.) 28, 524–538 (1958).
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The authors wish to express their gratitude to Dr. Sina Rosenthal, Department of Physiological Chemistry, Humboldt University, Berlin, who prepared the isolated membranes, to Mr. E. Fischer, Head Technician of the Department of Electron Microscopy, Greifswald University, who took most of the electron micrographs, to Mr. G. Bartsch, Department of Electron Microscopy, Greifswald University, and especially to Prof. W. Bargmann and to Doz. E. Lindner, Department of Anatomy, Kiel University, for many valuable suggestions.
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Deutsch, K., Krause, W. Electron microscopic observations on isolated mitochondrial membranes, prepared by various histological methods. Zeitschrift für Zellforschung 73, 132–142 (1966). https://doi.org/10.1007/BF00348470
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DOI: https://doi.org/10.1007/BF00348470