Abstract
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1.
The method of culturing “whole” rat embryos (days 9.5–11.5 of gestation, i.e. at the early stage of organogenesis) as modified and standardized in our laboratory is presented;
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2.
We have succeeded in using bovine serum as culture medium instead of rat serum as recommended in the original procedure. Experimental conditions are described for obtaining reproducible results;
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3.
An improved scoring system was developed which, in connection with a computerized documentation, greatly facilitates the evaluation of the data.
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References
Anschütz M, Herken R, Neubert D (1981) Studies on embryotoxic effects of thallium using the whole-embryo-culture technique. In: Neubert D, Merker HJ (eds) Culture Techniques, Applicability for studies on prenatal differentiation and toxicity, Walter de Gruyter Berlin, New York, pp 57–66
Beck F (1981) The differentiation between teratogenic action on mother, fetal membranes and embryos using the whole-embryo-culture method. In: Neubert D, Merker HJ (eds) Culture Techniques, Applicability for studies on prenatal differentiation and toxicity, Walter de Gruyter, Berlin, New York, pp 37–41
Brown NA, Fabro S (1981) Quantitation of rat embryonic development in vitro: A morphological scoring system. Teratology 24: 65–78
Chatot CL, Klein NW, Piatek J, Pierro LJ (1980) Successful culture of rat embryos on human serum: Use in the detection of teratogens. Science 207: 1471–1473
Herken R, Anschütz M (1981) Differentiation of embryonic tissues in whole-embryo-cultures as compared to the development in vivo. In: Neubert D, Merker HJ (eds) Culture Techniques, Applicability for studies on prenatal differentiation and toxicity. Walter de Gruyter, Berlin, New York, pp 19–35
Klee-Trieschmann U, Kröger J, Neubert D (1984) Whole-embryo culture of rodents using bovine serum or chemically defined media. Teratology 29: 27 A
Klein NW, Plenefisch JD, Carey SW, Chatot CL, Clapper ML (1980) Evaluation of serum teratogenic activity using rat embryo culture. In: Neubert D, Merker HJ (eds) Culture Techniques, Applicability for studies on prenatal differentiation and toxicity. Walter de Gruyter, Berlin, New York, pp 67–81
Klug S, Lewandowski C, Neubert D (1984) Scoring system for computer aided evaluation of whole-embryos in culture (growth, development and abnormalities) ETS, 12th Conf. Veldhoven, Niederlande, Teratology (in press)
Lewandowski C, Klug S, Neubert D (1984) Whole-embryo-culture of rodents: Improvement through use of bovine serum, ETS, 12th Conf. Veldhoven, Niederlande Teratology (in press)
New DAT (1978) Whole embryo culture and the study of mammalian embryos during organogenesis. Biol Rev 53: 81–122
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Data presented in this paper are part of the doctoral theses of Stephan Klug and Constanze Lewandowski to be presented to the Fachbereich Veterinärmedizin, Freie Universität Berlin
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Klug, S., Lewandowski, C. & Neubert, D. Modification and standardization of the culture of early postimplantation embryos for toxicological studies. Arch Toxicol 58, 84–88 (1985). https://doi.org/10.1007/BF00348314
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DOI: https://doi.org/10.1007/BF00348314