Abstract
Pasteurella haemolytica leukotoxin is a pore-forming cytolysin which acts as a virulence factor in pasteurellosis of domestic ruminants. Leukocytes from cattle, sheep and goats are susceptible to leukotoxin-induced lysis; however, leukocytes from non-ruminant species so far tested are resistant to leukotoxin-induced lysis. Neutrophils obtained from three white-tailed deer, four Saiga antelope, an Addra gazelle, a Grant's gazelle and a Sable antelope were tested for susceptibility to the lytic effects of P. haemolytica leukotoxin using lactate dehydrogenase release. Results were compared to those obtained using neutrophils from a steer and cultured bovine lymphoma cells. Neutrophils obtained from all these ruminants, except the Addra gazelle, were susceptible to P. haemolytica leukotoxin. Individual variation among the Saiga and the deer did not appear to be due to the percentages of neutrophils or the percentage of contaminating erythrocytes in the cell preparations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aikimbaev, M.A., Martinevskii, I.L., Altukhov, A.A., Ivanov, S.I. and Surov, V.F., 1985. Isolation of Pasteurella haemolytica from Saiga antelopes (Saiga tartarica) between February and March 1984 in the Uralsk region of Kazakhstan (in Russian). Izvestiya Akademii Nauk Kazakhskoi SSR, Seriya Biologicheskgya, 4, 39–41
Allan, E.M., Gibbs, H.A., Wiseman, A. and Selman, I.E., 1985. Sequential lesions of bovine pneumonic pasteurellosis. Veterinary Record, 117, 438–442
Carter, G.R., 1967. Pasteurellosis: Pasteurella multocida and Pasteurella haemolytica. Advances in Veterinary Science and Comparative Medicine, 11, 321–378.
Clinkenbeard, K.D., Mosier, D.A. and Confer, A.W., 1989a. Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin. Infection and Immunity, 57, 420–425
Clinkenbeard, K.D., Mosier, D.A., Timko, A.L. and Confer, A.W., 1989b. Effects of Pasteurella haemolytica on cultured bovine lymphoma cells. American Journal of Veterinary Research, 50, 271–275
Clinkenbeard, K.D., Mosier, D.A. and Confer, A.W., 1989c. Effects of Pasteurella haemolytica leukotoxin on isolated bovine neutrophils. Toxicon, 27, 797–804
Foreyt, W.J., 1989. Fatal Pasteurella haemolytica pneumonia in bighorn sheep after direct contact with clinically normal domestic sheep. American Journal of Veterinary Research, 50, 341–344
Griner, L.A., 1983. Pathology of Zoo Animals, (Zoological Society of San Diego, San Diego), 495–574
Hayashidani, H., Honda, E., Nakamura, T., Mori, Y., Sawada, T. and Ogawa, M., 1988. Outbreak of pneumonia caused by Pasteurella haemolytica infection in Shiba goats in Japan. Japanese Journal of Veterinary Science, 50, 960–962
Henson, P.M. and Oades, Z.G., 1975. Stimulation of human neutrophils by soluble and insoluble immunoglobulin aggregates. Journal of Clinical Investigation, 56, 1053–1061
Heuschele, W.P., 1986. Infectious diseases. In: M.E. Fowler (ed.), Zoo and Wild Animal Medicine, 2nd edn., (Saunders, Philadelphia), 57–62
Mwangota, A.U., Muhammed, S.I. and Thomson, R.G., 1978. Serological type of Pasteurella haemolytica in Kenya. Cornell Veterinarian, 68, 84–93
O'Brien, J.K. and Duffus, W.P.H., 1987. Pasteurella haemolytica cytotoxin: relative susceptibility of bovine leucocytes. Veterinary Microbiology, 13, 321–324
Onderka, D.K., Rawluk, S.A. and Wishart, W.D., 1988. Susceptibility of Rocky Mountain bighorn sheep and domestic sheep to pneumonia induced by bighorn and domestic livestock strains of Pasteurella haemolytica. Canadian Journal of Veterinary Research, 52, 439–444
Shewen, P.E. and Wilkie, B.N., 1982. Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes. Infection and Immunity, 35, 91–94
Yates, W.D.G., 1982. A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. Canadian Journal of Comparative Medicine, 46, 225–263
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Confer, A.W., Simons, K.R., Barrie, M.T. et al. Effects of pasteurella haemolytica leukotoxin on neutrophils from white-tailed deer and several exotic ruminant species. Veterinary Research Communications 14, 175–180 (1990). https://doi.org/10.1007/BF00347735
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00347735