Abstract
The purpose of our research was to study the activity of the phagocytic unit and the microbicidal systems of neutrophils when exposed to inactivated toxins of E. coli. The study was conducted in white rats, in which after immunization with toxoid components of E. coli, blood was collected to study the functional activity of neutrophils. In the course of the experiment, it was found that E. coli toxins generally activate the phagocytic link of immunity in vaccinated rats. The preparation containing the aggregate Heat-Labile Enterotoxins, Heat-Stable Enterotoxins, Shiga-Like Enterotoxins of E. coli during the first day after administration stimulates phagocytic activity of neutrophilic granulocytes by 2 times or more compared to the use of toxins separately. The digesting and capturing ability of neutrophils is most pronounced on the third day of research, after which a decrease in the functional activity of cells occurs. The activity of the microbicidal neutrophil system is most pronounced over 24–72 h, which was reflected in the stimulating effect on oxygen-dependent systems (myeloperoxidase, chloroacetateesterase, NBT test) and the oxygen-dependent system – cationic proteins (to a lesser extent).
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The reported study was funded by RFBR and Administration of Krasnodar Region according to the research project № 19-416-233007.
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Tishchenko, A., Terekhov, V., Novikova, E., Koba, I. (2022). Effect of Inactivated Escherichia Coli Exotoxins on the Phagocytic Properties of Neutrophils. In: Muratov, A., Ignateva, S. (eds) Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East (AFE-2021). AFE 2021. Lecture Notes in Networks and Systems, vol 354. Springer, Cham. https://doi.org/10.1007/978-3-030-91405-9_3
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