Abstract
Lepidopteran primary spermatocytes are bipotential leading first to regular (eupyrene) and later to irregular (apyrene) meiotic divisions. The kinetics of the lysine-rich proteins during this dichotomous meiosis was studied using the fluorescent dye sulfoflavine. Throughout the spermatogonial divisions, the chromatin fluoresces while the cytoplasm remains unstained. Reversely, during the meiotic prophase, the cytoplasm fluoresces strongly while the nuclei show only a few weakly fluorescing structures. From premetaphase to telophase the meiotic chromosomes fluoresce strongly again. But during this period, only in the eupyrene cells the cytoplasm remains strongly fluorescent; the fluorescence vanishs in the cytoplasm of the apyrene spermatocytes. Thus, the regular (eupyrene) meiotic divisions and the presence of a lysine-rich protein fraction in the cytoplasm of the dividing spermatocytes of Lepidoptera, are probably related.
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References
Bloch, D.P.: Cytochemistry of the histories. Protoplasmatologia 5/3a-d, 1–56 (1966)
Bogdanov, Yu.F., Strokov, A.A., Reznikova, S.A.: Histone synthesis during meiotic prophase of Lilium. Chromosoma (Berl.) 43, 237–245 (1973)
Brock, W.A., Trostle, P.K., Meistrich, M.L.: Meiotic synthesis of testes histones in the rat. Proc. nat. Acad. Sci. (Wash) 77, 371–375 (1980)
Carson, H.L.: A comparative study of the apical cells of the insect testis. J. Morph. 77, 141–161 (1941)
Friedländer, M., Benz, G.: The eupyrene-apyrene dichotomous spermatogenesis of Lepidoptera. Organ culture study on the timing of apyrene commitment in the codling moth. Int. J. Invertebr. Reprod. 3, 113–120 (1981)
Friedländer, M., Jans, P., Benz, G.: Precocius reprogramming of eupyrene-apyrene spermatogenesis commitment induced by allatectomy of the penultimate larval instar of the moth Actias selene. J. Insect. Physiol. 27, 267–269 (1981)
Friedländer, M., Miesel, S.: Spermatid anucleation during the normal atypical spermiogenesis of the warehouse moth Ephestia cautella. J. submicros. Cytol. 9, 173–185 (1977)
Friedländer, M., Wahrman, J.: The spindle as a basal body distributor. A study in the meiosis of the male silkworm moth, Bombyx mori. J. Cell Sci. 7, 65–89 (1970)
Hauschteck-Jungen, E., Hartl, D.L.: DNA distribution in spermatid nuclei of normal and segregation distorter males of Drosophila melanogaster. Genetics 89, 15–35 (1978)
Hauschteck-Jungen, E., Hartl, D.L.: Defective histone transition in heterozygous segregation distorted males of Drosophila melanogaster. Genetics (in press, 1982)
Hauschteck-Jungen, E., Maurer, B.: Sperm dysfunction in sex ratio males of Drosophila subobscura. Genetics 46, 459–477 (1976)
Kaye, J.S., McMaster-Kaye, R., Moss, S.B.: Fraction of cricket testis nuclei on gradients of colloidal silica for study of basic protein changes during spermiogenesis. Exp. Cell Res. 117, 245–252 (1978)
Leemann, U., Ruch, F.: Cytofluorometric determination of basic and total proteins with sulfaflavine. J. Histochem. Cytochem. 20, 659–671 (1972)
Leviatan, R., Friedländer, M.: The eupyrene-apyrene dichotomous spermatogenesis of Lepidoptera. I. The relationship with postembryonic development and the role of the decline in juvenile hormone titer towards pupation. Develop. Biol. 68, 515–524 (1979)
McMaster-Kaye, R., Kaye, J.S.: Organization of chromatin during spermiogenesis: beaded fibers, partially beaded fibers and loss of nucleosomal structure. Chromosoma (Berl.) 77, 41–56 (1980)
Meistrich, M.L., Brock, W.A., Grimes, S.Y., Platz, R.D., Hnilika, L.S.: Nuclear protein transition during spermatogenesis. Fed. Proc. 37, 2522–2525 (1978)
Meves, F.: Über oligopyrene und apyrene Spermien und über ihre Entstehung, nach Beobachtungen an Paludina und Pygaera. Arch, mikrosk. Anat. Entw. Mech. 61, 1–82 (1903)
Ruch, F.: Principles and some applications of cytofluorometry. In: Introduction to quantitative cytochemistry (G.L. Wied and G.F. Bahr, eds.), vol. 2, pp 431–450. New York: Academic Press 1970
Rüngger-Brändle, E., Jamrich, M., Bautz, E.K.F.: Localization of RNA polymerase B and histones in the nucleus of primary spermatocytes of Drosophila hydei, studied by immunofluorescence microscopy. Chromosoma (Berl.) 82, 388–407 (1981)
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Friedländer, M., Hauschteck-Jungen, E. The regular divisions of the spermatocytes as related to a meiotic lysine-rich protein fraction. Chromosoma 85, 109–118 (1982). https://doi.org/10.1007/BF00344597
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DOI: https://doi.org/10.1007/BF00344597