Summary
A simple device sensitive to 0.0001 c. c. for measuring oxygen consumption of a few milligrams of tissue is described. It incorporates a compensation chamber of large ratio (300∶1) to the respiration chamber, and a side arm with mercury piston to manipulate the dimethylaniline droplet and serve as a built-in calibrating device. The apparatus is compact, easy to clean, and the readings are directly translatable into volume without correction formulae.
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Dixon, M.: Manometric Methods as Applied to the Measurement of Cell Respiration and Other Processes. Cambridge Univ. Press 1934.
Duryee, W. R.: The Relationship between Water Content and Oxygen Consumption of the Organism. Science (N. Y.) 75, 518–519 (1932).
Fenn, W. O.: The Gas Exchange of Nerve during Stimulation. Amer. J. Physiol. 80, 327–346 (1927).
—: A New Method for the Simultaneous Determination of Minute Amounts of Carbon Dioxide and Oxygen. Amer. J. Physiol. 84, 110–118 (1928).
Fraps, M.: Studies on Respiration and Glycolysis in Planaria. I. Methods and Certain Basic Factors in Respiration. Physiologic. Zool. 3, 242–270 (1930).
Gerard, R. W.: A Modified Warburg Apparatus and Some Applications. Amer. J. Physiol. 97, 522 (1931).
Gerard, R. W. and H. K. Hartline: Respiration due to Natural Nerve Impulses. A Method for Measuring Respiration. J. cellul. a. comp. Physiol. 4, 141–160 (1934).
Howland, R. B. and A. Bernstein: A Method for Determining the Oxygen Consumption of a Single Cell. J. gen. Physiol. 14, 339–348 (1931).
Kalmus, H.: Die Messung der Atmung, Gärung und CO2-Assimilation kleiner Organismen in der Kapillare. Z. vergl. Physiol. 7, 304–313 (1928).
Krüger, F.: Ein neuer Mikro-Respirationsapparat zur fortlaufenden manometrischen Bestimmung des respiratorischen Quotienten. Z. vergl. Physiol. 21 (1934).
Novy, F. G., H. R. Roehm and M. H. Soule: Microbic Respiration. I. The Compensation Manometer and Other Means for the Study of Respiration. J. inf. Dis. 36, 109–167 (1925).
Schmitt, R. O.: The Oxygen Consumption of Stimulated Nerve. Amer. J. Physiol. 104, 303–319 (1933).
Thunberg, T.: Ein Mikrospirometer. Skand. Arch. Physiol. (Berl. u. Lpz.) 17, 74–85 (1905).
Warburg, O.: Über den Stoffwechsel der Tumoren. Berlin: Julius Springer 1926.
Winterstein, H.: Ein Mikrorespirationsapparat. Z. biol. Techn. 3, 246–250 (1913).
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Developed in conjunction with a dissertation for the Ph. d. degree at Osborn Zoological Laboratory, Yale University.
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Duryee, W.R. A modified microrespirometer. Z. f. vergl. Physiologie. 23, 208–213 (1936). https://doi.org/10.1007/BF00344194
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DOI: https://doi.org/10.1007/BF00344194