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Vergleichende fluoreszenzmikroskopische und elektronenmikroskopische Untersuchungen am zentralen Nervensystem von Planorbarius corneus L. (Basommatophora)

Comparative fluorescence- and electron microscopic studies of the central nervous system of Planorbarius corneus L. (Basommatophora)

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Summary

The neurosecretory system of the snail Planorbarius corneus has been investigated by fluorescence and electron microscopy. With the fluorochrome Pseudoisocyanin the established neurosecretory system in the cerebral ganglia and single neurosecretory cells in the other ganglia show an intensive yellow fluorescence. Electron micrographs reveal the presence of electron dense granules (elementary granules) in the pericarya and the axons of neurones which have the same localisation in the ganglia as the pseudoisocyanin-positive cells. The fluorescence technique for biogenic amines produces yellow and green fluorescence within neurons and in the neuropil and nerves. The fluorescence obtained in determinable areas and neurones correlates well with the electron microscopic location of “dense-core” vesicles within the pericarya and axons of cells in even the same areas. It is discussed, that in this animal both types of cells are so-called “neurosecretory cells”, because the high content of elementary granules in the “peptidergic neurosecretory cells” and of “dense-core” vesicles in the “aminergic neurosecretory cells” is an indication for secretion of these products in neurohaemal areas (circulatory channels).

Zusammenfassung

Durch Vergleich fluoreszenz- und elektronenmikroskopischer Untersuchungen am zentralen Nervensystem von Planorbarius corneus L. wird nachgewiesen, daß in den Schlundringganglien Neurosekretzellen vorkommen (Nachweis mit Pseudoisocyaninchlorid), die mit Nervenzellen nicht identisch sind, die durch ihren hohen Gehalt an biogenen Aminen auffallen (Nachweis durch die Methode von Falck und Owman, 1965). Es können daher im Schlundring von Planorbarius corneus peptiderge und aminerge Neurosekretzellen unterschieden werden. Die PSC-positiven Neurosekretzellen enthalten elektronendichte Elementargrana und die aminergen Neurone „dense-core“ Vesikel. Der Nachweis biogener Amine in einigen Nervenzellen von Planorbarius corneus spicht für deren chemische Identität mit Transmittersubstanzen, ihre hohe Konzentration aber für eine Abgabe in die Körperflüssigkeit.

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Literatur

  • Bargmann, W., Lindner, E., Andres, K. H.: Über Synapsen an endokrinen Epithelzellen und die Definition sekretorischer Neurone. Untersuchungen am Zwischenlappen der Katzenhypophyse. Z. Zellforsch. 77, 282–298 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Baxter, M. J., Nisbet, R. H.: Features of the nervous system and heart of Archachatina revealed by the electron microscope and by electro-physiological recording. Proc. Malacol. Soc. Lond. 35, 167–177 (1963).

    Google Scholar 

  • Berry, C. F., Cottrell, G. A.: Neurosecretion in the Vena cava of the cephalopod Eledone cirrosa. Z. Zellforsch. 104, 107–115 (1970).

    Article  CAS  PubMed  Google Scholar 

  • Boer, H. H.: A cytological and cytochemical study of neurosecretory cells in Basommatophora, with particular reference to Lymnaea stagnalis L. Arch. néerl. Zool. 16, 313–386 (1965).

    Article  CAS  Google Scholar 

  • —, Douma, E., Koksma, J. M. A.: Electron microscope study of neurosecretory cells and neurohaemal organs in the pond snail Lymnaea stagnalis. Symp. zool. Soc. Lond. 22, 237–256 (1968).

    Google Scholar 

  • —, Slot, J. W., Andel, J. van: Electron microscopical and histochemical observations on the relation between medio-dorsal bodies and neurosecretory cells in the basommatophoran snails Lymnaea stagnalis, Ancylus fluviatilis, Australorbis glabratus and Planorbarius corneus. Z. Zellforsch. 87, 435–450 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Brink, M., Boer, H. H.: An electron microscopical investigation of the follicle gland (cerebral gland) and of some neurosecretory cells in the lateral lobe of the cerebral ganglion of the pulmonate gastropod Lymnaea stagnalis L. Z. Zellforsch. 79, 230–243 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Clark, R. B.: The posterior lobes of the brain of Nephthys and the mucus-glands of the prostomium. Quart. J. micr. Sci. 96, 545–565 (1955).

    Google Scholar 

  • Cottrell, G. A., Maser, M.: Subcellular localization of 5-hydroxytryptamine and substance X in molluscan ganglia. Comp. Biochem. Physiol. 20, 901–906 (1967).

    Article  CAS  Google Scholar 

  • Dahl, E., Falck, B., Mecklenburg, C. von, Myhrberg, H., Rosengren, E.: Neuronal localization of dopamine and 5-hydroxytryptamine in some mollusca. Z. Zellforsch. 71, 489–498 (1966).

    Article  CAS  PubMed  Google Scholar 

  • Durchon, M.: L'endocrinologie des Vers et des Mollusques. Paris: Masson & Cie. 1967.

    Google Scholar 

  • Fährmann, W.: Licht- und elektronenmikroskopische Untersuchungen des Nervensystems von Unio tumidus (Philipsson) unter besonderer Berücksichtigung der Neurosekretion. Z. Zellforsch. 54, 689–716 (1961).

    Article  Google Scholar 

  • Falck, B., Owman, Ch.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund. 2, No. 7, 5–18 (1965).

    Google Scholar 

  • Gabe, M.: Neurosecretion. Oxford: Pergamon Press 1966.

    Google Scholar 

  • Gersch, M.: Vergleichende Endokrinologie der wirbellosen Tiere. Leipzig: Akad. Verlagsges. Geest & Portig, 1964.

    Google Scholar 

  • Gerschenfeld, H. M.: Observations on the ultrastructure of synapses in some pulmonate molluscs. Z. Zellforsch. 60, 258–275 (1963).

    Article  CAS  PubMed  Google Scholar 

  • Hanneforth, W.: Struktur und Funktion von Synapsen und synaptischen Grana in Gastropodennerven. Z. vergl. Physiol. 49, 485–520 (1965).

    Article  Google Scholar 

  • Herkstra, G. P., Lever, J.: Some effects of ganglion-extirpations in Lymnaea stagnalis. Proc. kon. ned. Akad. Wet. C 63, 271–282 (1960).

    Google Scholar 

  • Joose, J.: Dorsal bodies and dorsal neurosecretory cells of the cerebral ganglia of Lymnaea stagnalis. Arch. néerl. Zool. 16, 1–103 (1964).

    Article  Google Scholar 

  • Kerkut, G. A., Sedden, C. B., Walker, R. J.: Uptake of Dopa and 5-hydroxytryptophan by monoamine-forming neurones in the brain of Helix aspersa. Comp. Biochem. Physiol. 23, 159–162 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Knowles, F., Bern, H. A.: The function of neurosecretion in endocrine regulation. Nature (Lond.) 210, 271–272 (1966).

    Article  CAS  Google Scholar 

  • Kuhlmann, D.: Neurosekretion bei Heliciden. Z. Zellforsch. 60, 909–932 (1963).

    Article  CAS  PubMed  Google Scholar 

  • —, Nolte, A.: Spermiogenese, Eireifung und Neurosekretion. Untersuchungen an der Weinbergschnecke Helix pomatia L. (Gastropoda). Z. wiss. Zool. 176, 271–286 (1967).

    Google Scholar 

  • Laduron, P., Potter, W. de, Belpaire, F.: Storage of labeled noradrenaline in lysosomes. Life sci. 5, 2085–2094 (1966).

    Article  CAS  Google Scholar 

  • Laviolette, P.: Etude cytologique et expérimentale de la régénération germinale après castration chez Arion rufus L. (Gastéropode pulmoné). Ann. Sc. Nat. Zool., 11e S., 16, 431–535 (1954).

    Google Scholar 

  • Mol, J. J. van: Phénomènes neurosécrétoires dans les ganglions cérébroides d'Arion rufus. C. R. Acad. Sci. (Paris) 250, 2280–2281 (1960).

    Google Scholar 

  • —: Etude morphologique et phylogénétique du ganglion cérébroide des Gastéropodes Pulmonés (Mollusques). Acad. Roy. Belgique. Cl. Sci. 37, 1–68 (1967).

    Google Scholar 

  • Nolte, A.: Ultrastruktur des „Neurosekretmantels“ des Nervus labialis medius von Planorbarius corneus L. (Basommatophora). Naturwissenschaften 51, 148 (1964).

    Article  Google Scholar 

  • —: Neurohämal-„Organe“ bei Pulmonaten (Gastropoda). Zool. Jb., Abt. Anat. u. Ontog. 82, 365–380 (1965).

    Google Scholar 

  • - The mode of release of neurosecretory material in the freshwater pulmonate Lymnaea stagnalis L. (Gastropoda). Symp. Neurobiol. Invert. (Hung. Acad. Sci.), 123–133 (1967).

  • —, Breucker, H., Kuhlmann, D.: Cytosomale Einschlüsse und Neurosekret im Nervengewebe von Gastropoden. Z. Zellforsch. 68, 1–27 (1965).

    Article  CAS  PubMed  Google Scholar 

  • Pearse, A. G. E.: Histochemistry vol. 1. London: Churchill Ltd., 1968.

    Google Scholar 

  • Pelluet, D.: On the hormonal control of cell differentiation in the ovotestis of slugs (Gastropoda Pulmonata). Canad. J. Zool. 42, 195–199 (1964).

    Article  CAS  Google Scholar 

  • —, Lane, N. J.: The relation between neurosecretion and cell differentiation in the ovotestis of slugs (Gastropoda: Pulmonata). Canad. J. Zool. 39, 789–805 (1961).

    Article  CAS  Google Scholar 

  • Quattrini, D.: Dati preliminari di microscopia elettronica dei neuroni centrali di Vaginulus borellianus (Colosi), nel quadro del problema della neurosecrezione nei molluschi gasteropodi. Monit. zool. ital. 72, 3–12 (1964).

    Google Scholar 

  • —: Osservazioni al microscopia elettronica su un gruppo di neuroni centrali secernenti di Milax gagates Draparnaud (Mollusca Gastropoda Pulmonata). Boll. Soc. ital. Biol. sper. 41, 475–478 (1965).

    CAS  PubMed  Google Scholar 

  • Röhnisch, S.: Untersuchungen zur Neurosekretion bei Planorbarius corneus L. (Basommatophora). Z. Zellforsch. 63, 767–798 (1964).

    Article  PubMed  Google Scholar 

  • Rosenbluth, J.: The visceral ganglion of Aplysia californica. Z. Zellforsch. 60, 213–236 (1963).

    Article  CAS  PubMed  Google Scholar 

  • Sakharov, D. A., Borovyagin, V. L., Zs.-Nagy, I.: Light, fluorescence and electron microscopic studies on “neurosecretion” in Tritonia diomedia Bergh (Mollusca, Nudibranchia). Z. Zellforsch. 68, 660–673 (1965).

    Article  CAS  PubMed  Google Scholar 

  • —, Zs.-Nagy, I.: Localization of biogenic monoamines in cerebral ganglia of Lymnaea stagnalis L. Acta biol. Acad. Sci. hung. 19 (2), 145–157 (1968).

    CAS  Google Scholar 

  • Scharrer, B.: Neurohumors and neurohormones: Definitions and terminology. J. Neuro-Visceral Rel., Suppl. 9, 1–20 (1969).

    Google Scholar 

  • Schlote, F. W.: Neurosekretartige Grana in den peripheren Nerven und in den Nerv-Muskel-Verbindungen von Helix pomatia. Z. Zellforsch. 60, 325–347 (1963).

    Article  CAS  PubMed  Google Scholar 

  • Simpson, L.: Morphological studies of possible neuroendocrine structures in Helisoma tenue (Gastropoda: Pulmonata). Z. Zellforsch. 102, 570–593 (1969).

    Article  CAS  PubMed  Google Scholar 

  • —, Bern, H., Nishioka, R. S.: Examination of the evidence for neurosecretion in the nervous system of Helisoma tenue (Gastropoda: Pulmonata). Gen. comp. Endocr. 7, 525–548 (1966).

    Article  CAS  PubMed  Google Scholar 

  • Simpson, L., Bern, H., Nishioka, R. S.: Survey of evidence for neurosecretion in gastropod molluscs. Am. Zoologist 6, 123–138 (1966).

    Article  CAS  Google Scholar 

  • Vicente, N.: Histophysiologie du système nerveux des Gastéropodes Opisthobranches. Phénomènes neurosécrétoires. Rec. Trav. Stat. Mar. Endoume, Bull. 46, Fasc. 62, 13–121 (1969).

    Google Scholar 

  • Williams, E. E.: The effect of ganglion extirpation on 14C leucine incorporation in Littorina littorea (L.) (Mollusca, Prosobranchia). Comp. Biochem. Physiol. 33, 893–899 (1970).

    Article  Google Scholar 

  • Wohlfarth-Bottermann, K. E.: Die Kontrastierung tierischer Zellen und Gewebe im Rahmen ihrer elektronenmikroskopischen Untersuchung an ultradünnen Schnitten. Naturwissenschaften 44, 287–288 (1957).

    Article  Google Scholar 

  • Zimmermann, P.: Fluoreszenzmikroskopische Studien über die Verteilung und Regeneration der Faserglia bei Lumbricus terrestris L. Z. Zellforsch. 81, 190–220 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Zs.-Nagy, I.: Fluorescent-microscopic examination with Pseudoisocyanin on the neurosecretory activities of the fresh water mussel Anodonta cygnea L. Annal. Biol. Tihany 32, 123–127 (1965).

    Google Scholar 

  • —, S.-Rósza, K., Salanki, J., Földes, J., Perényi, L., Demeter, M.: Subcellular localization of 5-hydroxytryptamine in the central nervous system of Lamellibranchiates. J. Neurochem. 12, 245–251 (1965).

    Article  CAS  Google Scholar 

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  1. Abteilung Histophysiologie, Zoologisches Institut der Universität Münster, Deutschland

    Dieter Kühlmann

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Kühlmann, D. Vergleichende fluoreszenzmikroskopische und elektronenmikroskopische Untersuchungen am zentralen Nervensystem von Planorbarius corneus L. (Basommatophora). Z. Zellforsch. 110, 131–152 (1970). https://doi.org/10.1007/BF00343990

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